TY - JOUR
T1 - Control of initiation of pMB1 replication
T2 - Purified rop protein and RNA I affect primer formation in vitro
AU - Lacatena, R. M.
AU - Banner, D. W.
AU - Castagnoli, L.
AU - Cesareni, G.
PY - 1984
Y1 - 1984
N2 - We show that a protein of 63 amino acids is the product of the rop gene, a gene which negatively regulates the copy number of plasmids of the CoIE1 family. Rop protein purified to homogeneity inhibits CoIE1 plasmid replication in a bacterial extract. Furthermore, we show that Rop inhibition requires RNA I. In a purified in vitro system that can support primer transcription and processing, Rop affects primer formation in two ways: first, it elicits transcription termination at nucleotide 220, and second, it increases the ability of RNA I to inhibit RNAase H processing of the primer. The analysis of these data and the comparison with the results obtained in vivo with transcription fusion experiments allow us to propose a tentative model of the molecular mechanism underlying Rop-RNA I inhibition.
AB - We show that a protein of 63 amino acids is the product of the rop gene, a gene which negatively regulates the copy number of plasmids of the CoIE1 family. Rop protein purified to homogeneity inhibits CoIE1 plasmid replication in a bacterial extract. Furthermore, we show that Rop inhibition requires RNA I. In a purified in vitro system that can support primer transcription and processing, Rop affects primer formation in two ways: first, it elicits transcription termination at nucleotide 220, and second, it increases the ability of RNA I to inhibit RNAase H processing of the primer. The analysis of these data and the comparison with the results obtained in vivo with transcription fusion experiments allow us to propose a tentative model of the molecular mechanism underlying Rop-RNA I inhibition.
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U2 - 10.1016/0092-8674(84)90435-5
DO - 10.1016/0092-8674(84)90435-5
M3 - Article
C2 - 6204765
AN - SCOPUS:0021457138
VL - 37
SP - 1009
EP - 1014
JO - Cell
JF - Cell
SN - 0092-8674
IS - 3
ER -