Control of interleukin-18 secretion by dendritic cells: Role of calcium influxes

Stefania Gardella, Cristina Andrei, Alessandro Poggi, M. Raffaella Zocchi, Anna Rubartelli

Research output: Contribution to journalArticle

Abstract

Here we show that dendritic cells accumulate the precursor form of the leaderless secretory protein interleukin-18 (pro-interleukin-18) in the cell cytosol and in organelles co-fractionating with endolysosomes. Upon antigen specific contact with T lymphocytes, particulated pro-interleukin-18 decreases rapidly, and the cytokine appears extracellularly, suggesting that exocytosis of pro-interleukin-18-containing organelles is induced. Exocytosis of secretory lysosomes is modulated by calcium: in agreement with this, calcium influx results in secretion of pro-interleukin-18. In turn, pro- interleukin-18 secretion induced by T cells is prevented by the calcium channel blocker nifedipine. Our results demonstrate a novel, calcium-mediated mechanism of post-translational regulation of secretion for interleukin-18, that allows a fast release of the cytokine. (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)245-248
Number of pages4
JournalFEBS Letters
Volume481
Issue number3
DOIs
Publication statusPublished - Sep 22 2000

Keywords

  • Calcium channel
  • Degradation
  • Dendritic cell
  • Interleukin-18
  • Leaderless secretion
  • Secretory lysosome

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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