Control of Mitochondrial Remodeling by the ATPase Inhibitory Factor 1 Unveils a Pro-survival Relay via OPA1

Danilo Faccenda, Junji Nakamura, Giulia Gorini, Gurtej K. Dhoot, Mauro Piacentini, Masusuke Yoshida, Michelangelo Campanella

Research output: Contribution to journalArticle

Abstract

The ubiquitously expressed ATPase inhibitory factor 1 (IF1) is a mitochondrial protein that blocks the reversal of the F1Fo-ATPsynthase, preventing dissipation of cellular ATP and ischemic damage. IF1 suppresses programmed cell death, enhancing tumor invasion and chemoresistance, and is expressed in various types of human cancers. In this study, we examined its effect on mitochondrial redox balance and apoptotic cristae remodeling, finding that, by maintaining ATP levels, IF1 reduces glutathione (GSH) consumption and inactivation of peroxiredoxin 3 (Prx3) during apoptosis. This correlates with inhibition of metallopeptidase OMA1-mediated processing of the pro-fusion dynamin-related protein optic atrophy 1 (OPA1). Stabilization of OPA1 impedes cristae remodeling and completion of apoptosis. Taken together, these data suggest that IF1 acts on both mitochondrial bioenergetics and structure, is involved in mitochondrial signaling in tumor cells, and may underlie their proliferative capacity.

Original languageEnglish
Pages (from-to)1869-1883
Number of pages15
JournalCell Reports
Volume18
Issue number8
DOIs
Publication statusPublished - Feb 21 2017

Keywords

  • cancer
  • IF
  • mitochondria
  • OMA1
  • OPA1
  • Prxs
  • ROS

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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