Control of neuron outgrowth by NMDA receptors

R. Cuppini, S. Sartini, P. Ambrogini, E. Falcieri, M. C. Maltarello, G. Gallo

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The role of N-methyl-D-aspartic acid receptors (NMDARs) of glutamate on neuritogenesis was studied in cultured neurons of chick embryo spinal cord using the NMDAR non-competitive antagonist dizocilpine maleate (MK-801). No cell population was fully prevented from neuritogenesis by MK-801. Different aspects of neuritogenesis were quantitatively evaluated. Neurite initiation, elongation and branching were depressed by MK-801. Inhibition was dose-dependent and reversible. A loss of responsiveness of neuritogenesis to MK-801 was found during the second day of treatment at a concentration of 10 μM, but not at higher concentrations. Our findings support the idea that Ca2+ influx through NMDAR associated channels is one of the possible triggers of a cascade resulting in neuritogenesis. The effects of NMDAR blocking on neuritogenesis occurred before synaptogenesis, suggesting a role of excitatory aminoacids in neuron morphological differentiation at early stages of development. Scanning electron microscopy confirmed a reduction in neurite tree complexity in MK-801 treated cells and showed a production of filopodium-like processes in some of these cells.

Original languageEnglish
Pages (from-to)31-40
Number of pages10
JournalJournal of Submicroscopic Cytology and Pathology
Volume31
Issue number1
Publication statusPublished - Jan 1999

Fingerprint

Dizocilpine Maleate
N-Methyl-D-Aspartate Receptors
N-Methylaspartate
Neurons
Neurites
Pseudopodia
Chick Embryo
Electron Scanning Microscopy
Glutamic Acid
Spinal Cord
aspartic acid receptor
Population

Keywords

  • Chick embryo
  • Glutamate
  • MK-801
  • Neuritogenesis
  • Neuron
  • NMDAR

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Pathology and Forensic Medicine

Cite this

Cuppini, R., Sartini, S., Ambrogini, P., Falcieri, E., Maltarello, M. C., & Gallo, G. (1999). Control of neuron outgrowth by NMDA receptors. Journal of Submicroscopic Cytology and Pathology, 31(1), 31-40.

Control of neuron outgrowth by NMDA receptors. / Cuppini, R.; Sartini, S.; Ambrogini, P.; Falcieri, E.; Maltarello, M. C.; Gallo, G.

In: Journal of Submicroscopic Cytology and Pathology, Vol. 31, No. 1, 01.1999, p. 31-40.

Research output: Contribution to journalArticle

Cuppini, R, Sartini, S, Ambrogini, P, Falcieri, E, Maltarello, MC & Gallo, G 1999, 'Control of neuron outgrowth by NMDA receptors', Journal of Submicroscopic Cytology and Pathology, vol. 31, no. 1, pp. 31-40.
Cuppini R, Sartini S, Ambrogini P, Falcieri E, Maltarello MC, Gallo G. Control of neuron outgrowth by NMDA receptors. Journal of Submicroscopic Cytology and Pathology. 1999 Jan;31(1):31-40.
Cuppini, R. ; Sartini, S. ; Ambrogini, P. ; Falcieri, E. ; Maltarello, M. C. ; Gallo, G. / Control of neuron outgrowth by NMDA receptors. In: Journal of Submicroscopic Cytology and Pathology. 1999 ; Vol. 31, No. 1. pp. 31-40.
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