Control of stem cell proliferation and differentiation to achieve stable gene transfer and expression

F. Bertolini, M. Battaglia, A. Lanza, B. Palermo, G. Robustelli della Cuna

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Although the hematopoietic stem cell (HSC) seems to be a convenient target for gene therapy, data from human clinical trials have so far shown low levels of gene transduction. The new model of human stem cells, immunodeficient mice repopulating cells (SRC), has similarly demonstrated that SRC were rarely transduced by protocols used in past studies. Cytokines such as stem cell factor and interleukin-3, used so far to obtain cell proliferation in transduction protocols, might induce HSC to differentiate and impair their repopulating potential. In this scenario, there is a need for new gene transfer protocols associated with minimal cell differentiation and stable expression of the transduced gene in the majority of mature cells generated from transduced HSC.

Original languageEnglish
JournalBone Marrow Transplantation
Volume21
Issue numberSUPPL. 3
Publication statusPublished - 1998

Fingerprint

Hematopoietic Stem Cells
Cell Differentiation
Stem Cells
Cell Proliferation
Gene Expression
Stem Cell Factor
Interleukin-3
Genetic Therapy
Genes
Clinical Trials
Cytokines

Keywords

  • CD34
  • Gene transfer
  • Hematopoiesis
  • Stem cell

ASJC Scopus subject areas

  • Hematology
  • Transplantation

Cite this

Control of stem cell proliferation and differentiation to achieve stable gene transfer and expression. / Bertolini, F.; Battaglia, M.; Lanza, A.; Palermo, B.; Robustelli della Cuna, G.

In: Bone Marrow Transplantation, Vol. 21, No. SUPPL. 3, 1998.

Research output: Contribution to journalArticle

@article{dfeb631392c1481fa99173dc855b80b4,
title = "Control of stem cell proliferation and differentiation to achieve stable gene transfer and expression",
abstract = "Although the hematopoietic stem cell (HSC) seems to be a convenient target for gene therapy, data from human clinical trials have so far shown low levels of gene transduction. The new model of human stem cells, immunodeficient mice repopulating cells (SRC), has similarly demonstrated that SRC were rarely transduced by protocols used in past studies. Cytokines such as stem cell factor and interleukin-3, used so far to obtain cell proliferation in transduction protocols, might induce HSC to differentiate and impair their repopulating potential. In this scenario, there is a need for new gene transfer protocols associated with minimal cell differentiation and stable expression of the transduced gene in the majority of mature cells generated from transduced HSC.",
keywords = "CD34, Gene transfer, Hematopoiesis, Stem cell",
author = "F. Bertolini and M. Battaglia and A. Lanza and B. Palermo and {Robustelli della Cuna}, G.",
year = "1998",
language = "English",
volume = "21",
journal = "Bone Marrow Transplantation",
issn = "0268-3369",
publisher = "Nature Publishing Group",
number = "SUPPL. 3",

}

TY - JOUR

T1 - Control of stem cell proliferation and differentiation to achieve stable gene transfer and expression

AU - Bertolini, F.

AU - Battaglia, M.

AU - Lanza, A.

AU - Palermo, B.

AU - Robustelli della Cuna, G.

PY - 1998

Y1 - 1998

N2 - Although the hematopoietic stem cell (HSC) seems to be a convenient target for gene therapy, data from human clinical trials have so far shown low levels of gene transduction. The new model of human stem cells, immunodeficient mice repopulating cells (SRC), has similarly demonstrated that SRC were rarely transduced by protocols used in past studies. Cytokines such as stem cell factor and interleukin-3, used so far to obtain cell proliferation in transduction protocols, might induce HSC to differentiate and impair their repopulating potential. In this scenario, there is a need for new gene transfer protocols associated with minimal cell differentiation and stable expression of the transduced gene in the majority of mature cells generated from transduced HSC.

AB - Although the hematopoietic stem cell (HSC) seems to be a convenient target for gene therapy, data from human clinical trials have so far shown low levels of gene transduction. The new model of human stem cells, immunodeficient mice repopulating cells (SRC), has similarly demonstrated that SRC were rarely transduced by protocols used in past studies. Cytokines such as stem cell factor and interleukin-3, used so far to obtain cell proliferation in transduction protocols, might induce HSC to differentiate and impair their repopulating potential. In this scenario, there is a need for new gene transfer protocols associated with minimal cell differentiation and stable expression of the transduced gene in the majority of mature cells generated from transduced HSC.

KW - CD34

KW - Gene transfer

KW - Hematopoiesis

KW - Stem cell

UR - http://www.scopus.com/inward/record.url?scp=0031825930&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031825930&partnerID=8YFLogxK

M3 - Article

C2 - 9712501

AN - SCOPUS:0031825930

VL - 21

JO - Bone Marrow Transplantation

JF - Bone Marrow Transplantation

SN - 0268-3369

IS - SUPPL. 3

ER -