Copper activates the NF-κB pathway in vivo

Tiziana Persichini, Zulema Percario, Emanuela Mazzon, Marco Colasanti, Salvatore Cuzzocrea, Giovanni Musci

Research output: Contribution to journalArticle

Abstract

Previous results indicated that intravenous injection of copper in the form of a copper-histidine complex in rats triggers the transcriptional induction of the inducible form of nitric oxide synthase (NOS-II). Here, the authors demonstrate that copper activates the transcription factor NF-κB in the liver and lung tissues of rats, and that this effect is mediated by oxidative stress, since all copper-induced changes, which include histological alterations, formation of nitrotyrosines, vascular pressure drop, production of tumor necrosis factor-α (TNF-α), induction of NOS-II and nitrites, are readily prevented by pretreatment of the animals with the antioxidant tempol. By using electrophoretic mobility shift assays, the p50/p65 dimer and higher molecular weight aggregates have been found to be involved in the copper-induced NF-κB activation. COX-2, a NF-κB-dependent gene involved in the inflammatory response, was also transcriptionally induced by copper, this effect being reduced in the presence of tempol. These results suggest that a physiopathological status, characterized by hypercupremic situations, may lead to the onset of inflammation through production of ROS and activation of NF-κB.

Original languageEnglish
Pages (from-to)1897-1904
Number of pages8
JournalAntioxidants and Redox Signaling
Volume8
Issue number9-10
DOIs
Publication statusPublished - Sep 2006

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Copper
Nitric Oxide Synthase Type II
Rats
Chemical activation
Electrophoretic mobility
Oxidative stress
Electrophoretic Mobility Shift Assay
Nitrites
Nitric Oxide Synthase
Intravenous Injections
Dimers
Liver
Pressure drop
Blood Vessels
Assays
Animals
Oxidative Stress
Transcription Factors
Tumor Necrosis Factor-alpha
Antioxidants

ASJC Scopus subject areas

  • Biochemistry

Cite this

Persichini, T., Percario, Z., Mazzon, E., Colasanti, M., Cuzzocrea, S., & Musci, G. (2006). Copper activates the NF-κB pathway in vivo. Antioxidants and Redox Signaling, 8(9-10), 1897-1904. https://doi.org/10.1089/ars.2006.8.1897

Copper activates the NF-κB pathway in vivo. / Persichini, Tiziana; Percario, Zulema; Mazzon, Emanuela; Colasanti, Marco; Cuzzocrea, Salvatore; Musci, Giovanni.

In: Antioxidants and Redox Signaling, Vol. 8, No. 9-10, 09.2006, p. 1897-1904.

Research output: Contribution to journalArticle

Persichini, T, Percario, Z, Mazzon, E, Colasanti, M, Cuzzocrea, S & Musci, G 2006, 'Copper activates the NF-κB pathway in vivo', Antioxidants and Redox Signaling, vol. 8, no. 9-10, pp. 1897-1904. https://doi.org/10.1089/ars.2006.8.1897
Persichini T, Percario Z, Mazzon E, Colasanti M, Cuzzocrea S, Musci G. Copper activates the NF-κB pathway in vivo. Antioxidants and Redox Signaling. 2006 Sep;8(9-10):1897-1904. https://doi.org/10.1089/ars.2006.8.1897
Persichini, Tiziana ; Percario, Zulema ; Mazzon, Emanuela ; Colasanti, Marco ; Cuzzocrea, Salvatore ; Musci, Giovanni. / Copper activates the NF-κB pathway in vivo. In: Antioxidants and Redox Signaling. 2006 ; Vol. 8, No. 9-10. pp. 1897-1904.
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