Copper exposure effects on yeast mitochondrial proteome.

Lucia Banci, Ivano Bertini, Simone Ciofi-Baffoni, Annamaria D'Alessandro, Deepa Jaiswal, Valeria Marzano, Sara Neri, Maurizio Ronci, Andrea Urbani

Research output: Contribution to journalArticlepeer-review


Mitochondria play an important role on the entire cellular copper homeostatic mechanisms. Alteration of cellular copper levels may thus influence mitochondrial proteome and its investigation represents an important contribution to the general understanding of copper-related cellular effects. In these study we have performed an organelle targeted proteomic investigation focusing our attention on the effect of non-lethal 1mM copper concentration on Saccharomyces cerevisiae mitochondrial proteome. Functional copper effects on yeast mitochondrial proteome were evaluated by using both 2D electrophoresis (2-DE) and liquid chromatography coupled with tandem mass spectrometry. Proteomic data have been then analyzed by different unsupervised meta-analysis approaches that highlight the impairment of mitochondrial functions and the activation of oxidative stress response. Interestingly, our data have shown that stress response generated by 1mM copper treatment determines the activation of S. cerevisiae survival pathway. To investigate these findings we have treated yeast cells responsiveness to copper with hydrogen peroxide and observed a protective role of this metal. These results are suggestive of a copper role in the protection from oxidative stress possibly due to the activation of mechanisms involved in cellular survival and growth.

Original languageEnglish
Pages (from-to)2522-2535
Number of pages14
JournalJournal of Proteomics
Issue number11
Publication statusPublished - Oct 19 2011

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics


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