Abstract
Oxidative stress has been frequently implicated in the initiation and promotion phases of carcinogenesis. Antioxidant enzymes, which can antagonize this process, are lowered in a number of malignancies even though different findings have been reported in the literature. It has been shown that tumors have less copper/zinc superoxide dismutase (Cu/Zn SOD) in comparison with the more metabolically active tissues, but there is a large overlap between normal and tumor tissue. In order to examine the relationship between ostosarcoma at different degrees of proliferation and differentiation and Cu/ZN SOD levels, four different human osteosarcoma cell lines: HOS, U-2 OS, MG63, Saos-2 were studied for their production and release of Cu/ZN SOD. A normal human stromal cell line was used as control. Osteosarcoma cells were stimulated with TNF α, a cytokine previously shown to have antiproliferative activity. The release of Cu/ZN SOD into the supernatant was higher for the HOS and U-2 OS lines when compared to the other cell lines evaluated both in basal condition and after incubation with TNF α. Elevated intracellular levels of Cu/ZN SOD were shown except for the HOS and U-2 OS which possess high concentrations of the enzyme at 24 hours declining during the other incubation periods. These concentrations were increased after TNF α treatment. The different behaviour of the four cell lines evaluated might be explained by their degree of differentiation.
| Original language | English |
|---|---|
| Pages (from-to) | 1175-1180 |
| Number of pages | 6 |
| Journal | Anticancer Research |
| Volume | 18 |
| Issue number | 2 A |
| Publication status | Published - Mar 1998 |
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Keywords
- Osteosarcoma cells
- Oxgen antioxidant
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Copper/zinc superoxide dismutase expression by different human osteosarcoma cell lines. / Grigolo, Brunella; Lisignoli, Gina; Toneguzzi, Stefania; Mazzetti, Ilaria; Facchini, Andrea.
In: Anticancer Research, Vol. 18, No. 2 A, 03.1998, p. 1175-1180.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Copper/zinc superoxide dismutase expression by different human osteosarcoma cell lines
AU - Grigolo, Brunella
AU - Lisignoli, Gina
AU - Toneguzzi, Stefania
AU - Mazzetti, Ilaria
AU - Facchini, Andrea
PY - 1998/3
Y1 - 1998/3
N2 - Oxidative stress has been frequently implicated in the initiation and promotion phases of carcinogenesis. Antioxidant enzymes, which can antagonize this process, are lowered in a number of malignancies even though different findings have been reported in the literature. It has been shown that tumors have less copper/zinc superoxide dismutase (Cu/Zn SOD) in comparison with the more metabolically active tissues, but there is a large overlap between normal and tumor tissue. In order to examine the relationship between ostosarcoma at different degrees of proliferation and differentiation and Cu/ZN SOD levels, four different human osteosarcoma cell lines: HOS, U-2 OS, MG63, Saos-2 were studied for their production and release of Cu/ZN SOD. A normal human stromal cell line was used as control. Osteosarcoma cells were stimulated with TNF α, a cytokine previously shown to have antiproliferative activity. The release of Cu/ZN SOD into the supernatant was higher for the HOS and U-2 OS lines when compared to the other cell lines evaluated both in basal condition and after incubation with TNF α. Elevated intracellular levels of Cu/ZN SOD were shown except for the HOS and U-2 OS which possess high concentrations of the enzyme at 24 hours declining during the other incubation periods. These concentrations were increased after TNF α treatment. The different behaviour of the four cell lines evaluated might be explained by their degree of differentiation.
AB - Oxidative stress has been frequently implicated in the initiation and promotion phases of carcinogenesis. Antioxidant enzymes, which can antagonize this process, are lowered in a number of malignancies even though different findings have been reported in the literature. It has been shown that tumors have less copper/zinc superoxide dismutase (Cu/Zn SOD) in comparison with the more metabolically active tissues, but there is a large overlap between normal and tumor tissue. In order to examine the relationship between ostosarcoma at different degrees of proliferation and differentiation and Cu/ZN SOD levels, four different human osteosarcoma cell lines: HOS, U-2 OS, MG63, Saos-2 were studied for their production and release of Cu/ZN SOD. A normal human stromal cell line was used as control. Osteosarcoma cells were stimulated with TNF α, a cytokine previously shown to have antiproliferative activity. The release of Cu/ZN SOD into the supernatant was higher for the HOS and U-2 OS lines when compared to the other cell lines evaluated both in basal condition and after incubation with TNF α. Elevated intracellular levels of Cu/ZN SOD were shown except for the HOS and U-2 OS which possess high concentrations of the enzyme at 24 hours declining during the other incubation periods. These concentrations were increased after TNF α treatment. The different behaviour of the four cell lines evaluated might be explained by their degree of differentiation.
KW - Osteosarcoma cells
KW - Oxgen antioxidant
UR - http://www.scopus.com/inward/record.url?scp=0031812110&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031812110&partnerID=8YFLogxK
M3 - Article
VL - 18
SP - 1175
EP - 1180
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 2 A
ER -