Copper/zinc superoxide dismutase expression by different human osteosarcoma cell lines

Brunella Grigolo, Gina Lisignoli, Stefania Toneguzzi, Ilaria Mazzetti, Andrea Facchini

Research output: Contribution to journalArticlepeer-review

Abstract

Oxidative stress has been frequently implicated in the initiation and promotion phases of carcinogenesis. Antioxidant enzymes, which can antagonize this process, are lowered in a number of malignancies even though different findings have been reported in the literature. It has been shown that tumors have less copper/zinc superoxide dismutase (Cu/Zn SOD) in comparison with the more metabolically active tissues, but there is a large overlap between normal and tumor tissue. In order to examine the relationship between ostosarcoma at different degrees of proliferation and differentiation and Cu/ZN SOD levels, four different human osteosarcoma cell lines: HOS, U-2 OS, MG63, Saos-2 were studied for their production and release of Cu/ZN SOD. A normal human stromal cell line was used as control. Osteosarcoma cells were stimulated with TNF α, a cytokine previously shown to have antiproliferative activity. The release of Cu/ZN SOD into the supernatant was higher for the HOS and U-2 OS lines when compared to the other cell lines evaluated both in basal condition and after incubation with TNF α. Elevated intracellular levels of Cu/ZN SOD were shown except for the HOS and U-2 OS which possess high concentrations of the enzyme at 24 hours declining during the other incubation periods. These concentrations were increased after TNF α treatment. The different behaviour of the four cell lines evaluated might be explained by their degree of differentiation.

Original languageEnglish
Pages (from-to)1175-1180
Number of pages6
JournalAnticancer Research
Volume18
Issue number2 A
Publication statusPublished - Mar 1998

Keywords

  • Osteosarcoma cells
  • Oxgen antioxidant

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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