Abstract
Oxidative stress has been frequently implicated in the initiation and promotion phases of carcinogenesis. Antioxidant enzymes, which can antagonize this process, are lowered in a number of malignancies even though different findings have been reported in the literature. It has been shown that tumors have less copper/zinc superoxide dismutase (Cu/Zn SOD) in comparison with the more metabolically active tissues, but there is a large overlap between normal and tumor tissue. In order to examine the relationship between ostosarcoma at different degrees of proliferation and differentiation and Cu/ZN SOD levels, four different human osteosarcoma cell lines: HOS, U-2 OS, MG63, Saos-2 were studied for their production and release of Cu/ZN SOD. A normal human stromal cell line was used as control. Osteosarcoma cells were stimulated with TNF α, a cytokine previously shown to have antiproliferative activity. The release of Cu/ZN SOD into the supernatant was higher for the HOS and U-2 OS lines when compared to the other cell lines evaluated both in basal condition and after incubation with TNF α. Elevated intracellular levels of Cu/ZN SOD were shown except for the HOS and U-2 OS which possess high concentrations of the enzyme at 24 hours declining during the other incubation periods. These concentrations were increased after TNF α treatment. The different behaviour of the four cell lines evaluated might be explained by their degree of differentiation.
Original language | English |
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Pages (from-to) | 1175-1180 |
Number of pages | 6 |
Journal | Anticancer Research |
Volume | 18 |
Issue number | 2 A |
Publication status | Published - Mar 1998 |
Keywords
- Osteosarcoma cells
- Oxgen antioxidant
ASJC Scopus subject areas
- Cancer Research
- Oncology