TY - JOUR
T1 - COPZ1 depletion in thyroid tumor cells triggers type I IFN response and immunogenic cell death
AU - Di Marco, Tiziana
AU - Bianchi, Francesca
AU - Sfondrini, Lucia
AU - Todoerti, Katia
AU - Bongarzone, Italia
AU - Maffioli, Elisa Margherita
AU - Tedeschi, Gabriella
AU - Mazzoni, Mara
AU - Pagliardini, Sonia
AU - Pellegrini, Sandra
AU - Neri, Antonino
AU - Anania, Maria Chiara
AU - Greco, Angela
PY - 2020/4/28
Y1 - 2020/4/28
N2 - The coatomer protein complex zeta 1 (COPZ1) represents a non-oncogene addiction for thyroid cancer (TC); its depletion impairs the viability of thyroid tumor cells, leads to abortive autophagy, ER stress, UPR and apoptosis, and reduces tumor growth of TC xenograft models. In this study we investigated the molecular pathways activated by COPZ1 depletion and the paracrine effects on cellular microenvironment and immune response. By comprehensive and target approaches we demonstrated that COPZ1 depletion in TPC-1 and 8505C thyroid tumor cell lines activates type I IFN pathway and viral mimicry responses. The secretome from COPZ1-depleted cells was enriched for several inflammatory molecules and damage-associated molecular patterns (DAMPs). Moreover, we found that dendritic cells, exposed to these secretomes, expressed high levels of differentiation and maturation markers, and stimulated the proliferation of naïve T cells. Interestingly, T cells stimulated with COPZ1-depleted cells showed increased cytotoxic activity against parental tumor cells. Collectively, our findings support the notion that targeting COPZ1 may represent a promising therapeutic approach for TC, considering its specificity for cancer cells, the lack of effect on normal cells, and the capacity to prompt an anti-tumor immune response.
AB - The coatomer protein complex zeta 1 (COPZ1) represents a non-oncogene addiction for thyroid cancer (TC); its depletion impairs the viability of thyroid tumor cells, leads to abortive autophagy, ER stress, UPR and apoptosis, and reduces tumor growth of TC xenograft models. In this study we investigated the molecular pathways activated by COPZ1 depletion and the paracrine effects on cellular microenvironment and immune response. By comprehensive and target approaches we demonstrated that COPZ1 depletion in TPC-1 and 8505C thyroid tumor cell lines activates type I IFN pathway and viral mimicry responses. The secretome from COPZ1-depleted cells was enriched for several inflammatory molecules and damage-associated molecular patterns (DAMPs). Moreover, we found that dendritic cells, exposed to these secretomes, expressed high levels of differentiation and maturation markers, and stimulated the proliferation of naïve T cells. Interestingly, T cells stimulated with COPZ1-depleted cells showed increased cytotoxic activity against parental tumor cells. Collectively, our findings support the notion that targeting COPZ1 may represent a promising therapeutic approach for TC, considering its specificity for cancer cells, the lack of effect on normal cells, and the capacity to prompt an anti-tumor immune response.
KW - COPZ1
KW - Immunogenic cell death
KW - Inflammation
KW - Thyroid cancer
KW - Type I IFN
UR - http://www.scopus.com/inward/record.url?scp=85079620498&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85079620498&partnerID=8YFLogxK
U2 - 10.1016/j.canlet.2020.02.011
DO - 10.1016/j.canlet.2020.02.011
M3 - Article
C2 - 32061953
AN - SCOPUS:85079620498
VL - 476
SP - 106
EP - 119
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
ER -