Cord blood basophil releasability: Evaluation of histamine release and leukotriene c4 generation

Antonio Miadonna, Ennio Leggieri, Alberto Tedeschi, Maurizio Lorini, Giuliano Motta, Gina Lucci, Marco Froldi, Antonio Marini, Carlo Zanussi

Research output: Contribution to journalArticle

Abstract

This study was performed to evaluate mediator (histamine and leukotriene C4) release from cord blood and adult blood basophils, challenged with IgE-independent (calcium ionophore A23187) and IgE-mediated (anti-IgE) stimuli. IgE-independent mediator release was similar in adult blood and umbilical blood basophils. Conversely, the anti-IgE-induced histamine and immunoreactive leukotriene C4 (iLTC4) release was significantly reduced in cord blood basophils. Passive sensitization with an IgE-rich serum was followed by a significant increase in the number of eluted IgE molecules from cord blood basophils and by an increase in IgE-mediated histamine release. Iltc4 production was not affected by passive sensitization of umbilical blood basophils. The IgE-dependent mediator release from cord blood basophils was not correlated with the number of cell-bound IgE. In addition, histamine secretion and leukotriene C4 production from cord blood basophils seem to be independent events.

Original languageEnglish
Pages (from-to)42-47
Number of pages6
JournalInternational Archives of Allergy and Immunology
Volume86
Issue number1
DOIs
Publication statusPublished - 1988

Fingerprint

Leukotriene C4
Basophils
Histamine Release
Fetal Blood
Immunoglobulin E
Histamine
Umbilicus
Calcium Ionophores
Calcimycin
Cell Count

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Miadonna, A., Leggieri, E., Tedeschi, A., Lorini, M., Motta, G., Lucci, G., ... Zanussi, C. (1988). Cord blood basophil releasability: Evaluation of histamine release and leukotriene c4 generation. International Archives of Allergy and Immunology, 86(1), 42-47. https://doi.org/10.1159/000234604

Cord blood basophil releasability : Evaluation of histamine release and leukotriene c4 generation. / Miadonna, Antonio; Leggieri, Ennio; Tedeschi, Alberto; Lorini, Maurizio; Motta, Giuliano; Lucci, Gina; Froldi, Marco; Marini, Antonio; Zanussi, Carlo.

In: International Archives of Allergy and Immunology, Vol. 86, No. 1, 1988, p. 42-47.

Research output: Contribution to journalArticle

Miadonna, A, Leggieri, E, Tedeschi, A, Lorini, M, Motta, G, Lucci, G, Froldi, M, Marini, A & Zanussi, C 1988, 'Cord blood basophil releasability: Evaluation of histamine release and leukotriene c4 generation', International Archives of Allergy and Immunology, vol. 86, no. 1, pp. 42-47. https://doi.org/10.1159/000234604
Miadonna, Antonio ; Leggieri, Ennio ; Tedeschi, Alberto ; Lorini, Maurizio ; Motta, Giuliano ; Lucci, Gina ; Froldi, Marco ; Marini, Antonio ; Zanussi, Carlo. / Cord blood basophil releasability : Evaluation of histamine release and leukotriene c4 generation. In: International Archives of Allergy and Immunology. 1988 ; Vol. 86, No. 1. pp. 42-47.
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