Cord blood basophil releasability: Evaluation of histamine release and leukotriene c4 generation

Antonio Miadonna, Ennio Leggieri, Alberto Tedeschi, Maurizio Lorini, Giuliano Motta, Gina Lucci, Marco Froldi, Antonio Marini, Carlo Zanussi

Research output: Contribution to journalArticlepeer-review

Abstract

This study was performed to evaluate mediator (histamine and leukotriene C4) release from cord blood and adult blood basophils, challenged with IgE-independent (calcium ionophore A23187) and IgE-mediated (anti-IgE) stimuli. IgE-independent mediator release was similar in adult blood and umbilical blood basophils. Conversely, the anti-IgE-induced histamine and immunoreactive leukotriene C4 (iLTC4) release was significantly reduced in cord blood basophils. Passive sensitization with an IgE-rich serum was followed by a significant increase in the number of eluted IgE molecules from cord blood basophils and by an increase in IgE-mediated histamine release. Iltc4 production was not affected by passive sensitization of umbilical blood basophils. The IgE-dependent mediator release from cord blood basophils was not correlated with the number of cell-bound IgE. In addition, histamine secretion and leukotriene C4 production from cord blood basophils seem to be independent events.

Original languageEnglish
Pages (from-to)42-47
Number of pages6
JournalInternational Archives of Allergy and Immunology
Volume86
Issue number1
DOIs
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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