Cord blood-derived neuronal cells by ectopic expression of Sox2 and c-Myc

Alessandra Giorgetti, Maria C N Marchetto, Mo Li, Diana Yu, Raffaella Fazzina, Yangling Mu, Antonio Adamo, Ida Paramonov, Julio Castaño Cardoso, Montserrat Barragan Monasterio, Cedric Bardy, Riccardo Cassiani-Ingoni, Guang Hui Liu, Fred H. Gage, Juan Carlos Izpisua Belmonte

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The finding that certain somatic cells can be directly converted into cells of other lineages by the delivery of specific sets of transcription factors paves the way to novel therapeutic applications. Here we show that human cord blood (CB) CD133+ cells lose their hematopoietic signature and are converted into CB-induced neuronal- like cells (CB-iNCs) by the ectopic expression of the transcription factor Sox2, a process that is further augmented by the combination of Sox2 and c-Myc. Gene-expression analysis, immunophenotyping, and electrophysiological analysis show that CB-iNCs acquire a distinct neuronal phenotype characterized by the expression of multiple neuronal markers. CB-iNCs show the ability to fire action potentials after in vitro maturation as well as after in vivo transplantation into the mouse hippocampus. This system highlights the potential of CB cells and offers an alternative means to the study of cellular plasticity, possibly in the context of drug screening research and of future cell-replacement therapies.

Original languageEnglish
Pages (from-to)12556-12561
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number31
DOIs
Publication statusPublished - Jul 31 2012

Fingerprint

Fetal Blood
Blood Cells
Transcription Factors
Immunophenotyping
Preclinical Drug Evaluations
Cell Lineage
Cell- and Tissue-Based Therapy
Action Potentials
Hippocampus
Transplantation
Ectopic Gene Expression
Phenotype
Gene Expression
Research
Therapeutics

Keywords

  • Neurons
  • Reprogramming
  • Stem cells

ASJC Scopus subject areas

  • General

Cite this

Giorgetti, A., Marchetto, M. C. N., Li, M., Yu, D., Fazzina, R., Mu, Y., ... Belmonte, J. C. I. (2012). Cord blood-derived neuronal cells by ectopic expression of Sox2 and c-Myc. Proceedings of the National Academy of Sciences of the United States of America, 109(31), 12556-12561. https://doi.org/10.1073/pnas.1209523109

Cord blood-derived neuronal cells by ectopic expression of Sox2 and c-Myc. / Giorgetti, Alessandra; Marchetto, Maria C N; Li, Mo; Yu, Diana; Fazzina, Raffaella; Mu, Yangling; Adamo, Antonio; Paramonov, Ida; Cardoso, Julio Castaño; Monasterio, Montserrat Barragan; Bardy, Cedric; Cassiani-Ingoni, Riccardo; Liu, Guang Hui; Gage, Fred H.; Belmonte, Juan Carlos Izpisua.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 31, 31.07.2012, p. 12556-12561.

Research output: Contribution to journalArticle

Giorgetti, A, Marchetto, MCN, Li, M, Yu, D, Fazzina, R, Mu, Y, Adamo, A, Paramonov, I, Cardoso, JC, Monasterio, MB, Bardy, C, Cassiani-Ingoni, R, Liu, GH, Gage, FH & Belmonte, JCI 2012, 'Cord blood-derived neuronal cells by ectopic expression of Sox2 and c-Myc', Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 31, pp. 12556-12561. https://doi.org/10.1073/pnas.1209523109
Giorgetti, Alessandra ; Marchetto, Maria C N ; Li, Mo ; Yu, Diana ; Fazzina, Raffaella ; Mu, Yangling ; Adamo, Antonio ; Paramonov, Ida ; Cardoso, Julio Castaño ; Monasterio, Montserrat Barragan ; Bardy, Cedric ; Cassiani-Ingoni, Riccardo ; Liu, Guang Hui ; Gage, Fred H. ; Belmonte, Juan Carlos Izpisua. / Cord blood-derived neuronal cells by ectopic expression of Sox2 and c-Myc. In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 31. pp. 12556-12561.
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