Cord blood plasma-mediated ex vivo expansion of hematopoietic progenitor cells

F. Bertolini, L. Lazzari, E. Lauri, C. Corsini, G. Sirchia

Research output: Contribution to journalArticle

Abstract

Cord blood (CB) plasma has previously been found to augment the replating capacity of CB-derived hematopoietic progenitors. In the present study, we observed a 2.8 ± 0.3 and a 1.8 ± 0.2-fold expansion of CFU-GM in cultures of CB cells without growth factors but supplemented by CB plasma or maternal blood (MB) plasma, respectively. In the absence of growth factors, CFU-GM expansion did not occur in CB cell cultures supplemented with fetal calf serum (FCS), peripheral blood (PB) plasma, PB plasma plus concentrations of IL-6 similar to those previously reported in CB plasma and in bone marrow (BM) cell cultures supplemented with FCS, CB, MB or PB plasma. In the presence of stem cell factor (SCF), IL-3 and IL-11, an expansion of CFU-GM was observed in CB and BM cell cultures supplemented by either CB, MB or PB plasma or FCS. Nevertheless, progenitor expansion was significantly superior in CB cell cultures supplemented by CB plasma (8.7 ± 0.7, 2.4 ± 0.3 and 2.6 ± 0.3-fold expansions for CFU-GM, BFU-E and CFU-Mix, respectively). In conclusion, an unknown factor(s) present in CB plasma, probably capable of crossing the placenta, has an effect alone and in the presence of growth factors in supporting ex vivo expansion of CB progenitors.

Original languageEnglish
Pages (from-to)347-353
Number of pages7
JournalBone Marrow Transplantation
Volume14
Issue number3
Publication statusPublished - 1994

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Hematopoietic Stem Cells
Fetal Blood
Granulocyte-Macrophage Progenitor Cells
Cell Culture Techniques
Blood Cells
Intercellular Signaling Peptides and Proteins
Mothers
Bone Marrow Cells
Serum
Interleukin-11
Erythroid Precursor Cells
Stem Cell Factor
Interleukin-3
Placenta
Interleukin-6

ASJC Scopus subject areas

  • Hematology
  • Transplantation

Cite this

Cord blood plasma-mediated ex vivo expansion of hematopoietic progenitor cells. / Bertolini, F.; Lazzari, L.; Lauri, E.; Corsini, C.; Sirchia, G.

In: Bone Marrow Transplantation, Vol. 14, No. 3, 1994, p. 347-353.

Research output: Contribution to journalArticle

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AU - Lazzari, L.

AU - Lauri, E.

AU - Corsini, C.

AU - Sirchia, G.

PY - 1994

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AB - Cord blood (CB) plasma has previously been found to augment the replating capacity of CB-derived hematopoietic progenitors. In the present study, we observed a 2.8 ± 0.3 and a 1.8 ± 0.2-fold expansion of CFU-GM in cultures of CB cells without growth factors but supplemented by CB plasma or maternal blood (MB) plasma, respectively. In the absence of growth factors, CFU-GM expansion did not occur in CB cell cultures supplemented with fetal calf serum (FCS), peripheral blood (PB) plasma, PB plasma plus concentrations of IL-6 similar to those previously reported in CB plasma and in bone marrow (BM) cell cultures supplemented with FCS, CB, MB or PB plasma. In the presence of stem cell factor (SCF), IL-3 and IL-11, an expansion of CFU-GM was observed in CB and BM cell cultures supplemented by either CB, MB or PB plasma or FCS. Nevertheless, progenitor expansion was significantly superior in CB cell cultures supplemented by CB plasma (8.7 ± 0.7, 2.4 ± 0.3 and 2.6 ± 0.3-fold expansions for CFU-GM, BFU-E and CFU-Mix, respectively). In conclusion, an unknown factor(s) present in CB plasma, probably capable of crossing the placenta, has an effect alone and in the presence of growth factors in supporting ex vivo expansion of CB progenitors.

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