Cordycepin reduces the sensitivity of BALB/Mo mouse lymphocytes to the induction of sister chromatid exchanges

F. Majone, A. Montaldi, F. Ronchese, A. De Rossi, L. Chieco-Bianchi, A. G. Levis

Research output: Contribution to journalArticle

Abstract

Lymphocytes from the spleen of BALB/Mo mice, which carry endogenous Moloney murine leukemia virus (M-MuLV), show in vitro frequencies of sister chromatid exchanges (SCEs) significantly higher than lymphocytes from control (M-MuLV free) BALB/c mice. In vitro treatment of lymphocytes with the antiviral antibiotic cordycepin (10 μg/ml) lowers the level of SCEs in BALB/Mo cells to the same value of BALB/c cells. M-MuLV yield is also markedly reduced in BALB/Mo lymphocytes cultured in the presence of cordycepin. The drug also abolishes the increased sensitivity of BALB/Mo lymphocytes to the induction of SCEs by mitomycin C (MMC) either in vitro (3 x 10-8/10-7M) or in vivo (0.3/3 mg/kg). Since cordycepin is known to inhibit poly(A)synthesis thus blocking RNA maturation, it is suggested that M-MuLV proviral integration is not per se the sole factor responsible for the more pronounced susceptibility of BALB/Mo lymphocytes to SCE induction, but most likely viral gene expression and amplification are needed for this effect of occur.

Original languageEnglish
Pages (from-to)131-134
Number of pages4
JournalCarcinogenesis
Volume6
Issue number1
Publication statusPublished - 1985

ASJC Scopus subject areas

  • Cancer Research
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience

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    Majone, F., Montaldi, A., Ronchese, F., De Rossi, A., Chieco-Bianchi, L., & Levis, A. G. (1985). Cordycepin reduces the sensitivity of BALB/Mo mouse lymphocytes to the induction of sister chromatid exchanges. Carcinogenesis, 6(1), 131-134.