Corneal Bioengineering

Francesca Corradini, Michela Zattoni, Paolo Rama, Michele De Luca, Graziella Pellegrini

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The cornea is the main structure of the ocular surface and enables the transmission of light entering the eye. The cornea is covered by a nonkeratinized stratified epithelium, which is renewed by stem cells located in the basal layer of the limbus. Injury and disease, such as chemical and thermal burns, can destroy the limbus leading to a limbal stem cell deficiency (LSCD) with consequent visual loss. In 1997, Pellegrini et al. firstly described the use of ex vivo cultured limbal epithelial stem cell to treat LSCD. Since then, several reports describing alternative methods of the clinical use of this technology have been published but the retention of stem cells, which is the essential property of the graft, has not been investigated. The definition of a graftable limbal culture in light of the clinical performance must follow specific quality criteria, here discussed, which are relevant for the future use of any cultured cell type for clinical application.

Original languageEnglish
Title of host publicationRegenerative Medicine Applications in Organ Transplantation
PublisherElsevier Inc.
Pages829-840
Number of pages12
ISBN (Print)9780123985231
DOIs
Publication statusPublished - 2014

Fingerprint

Bioengineering
Stem cells
Stem Cells
Cornea
Chemical Burns
Grafts
Cultured Cells
Epithelium
Hot Temperature
Epithelial Cells
Cells
Technology
Transplants
Light
Wounds and Injuries

Keywords

  • Cell therapy
  • Cornea
  • Holoclone
  • Keratinocytes
  • Limbus
  • Regenerative medicine
  • Squamous epithelia
  • Tissue engineering

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Corradini, F., Zattoni, M., Rama, P., De Luca, M., & Pellegrini, G. (2014). Corneal Bioengineering. In Regenerative Medicine Applications in Organ Transplantation (pp. 829-840). Elsevier Inc.. https://doi.org/10.1016/B978-0-12-398523-1.00059-8

Corneal Bioengineering. / Corradini, Francesca; Zattoni, Michela; Rama, Paolo; De Luca, Michele; Pellegrini, Graziella.

Regenerative Medicine Applications in Organ Transplantation. Elsevier Inc., 2014. p. 829-840.

Research output: Chapter in Book/Report/Conference proceedingChapter

Corradini, F, Zattoni, M, Rama, P, De Luca, M & Pellegrini, G 2014, Corneal Bioengineering. in Regenerative Medicine Applications in Organ Transplantation. Elsevier Inc., pp. 829-840. https://doi.org/10.1016/B978-0-12-398523-1.00059-8
Corradini F, Zattoni M, Rama P, De Luca M, Pellegrini G. Corneal Bioengineering. In Regenerative Medicine Applications in Organ Transplantation. Elsevier Inc. 2014. p. 829-840 https://doi.org/10.1016/B978-0-12-398523-1.00059-8
Corradini, Francesca ; Zattoni, Michela ; Rama, Paolo ; De Luca, Michele ; Pellegrini, Graziella. / Corneal Bioengineering. Regenerative Medicine Applications in Organ Transplantation. Elsevier Inc., 2014. pp. 829-840
@inbook{b30c8bfc48674bbfb2149b75c6f5f3cb,
title = "Corneal Bioengineering",
abstract = "The cornea is the main structure of the ocular surface and enables the transmission of light entering the eye. The cornea is covered by a nonkeratinized stratified epithelium, which is renewed by stem cells located in the basal layer of the limbus. Injury and disease, such as chemical and thermal burns, can destroy the limbus leading to a limbal stem cell deficiency (LSCD) with consequent visual loss. In 1997, Pellegrini et al. firstly described the use of ex vivo cultured limbal epithelial stem cell to treat LSCD. Since then, several reports describing alternative methods of the clinical use of this technology have been published but the retention of stem cells, which is the essential property of the graft, has not been investigated. The definition of a graftable limbal culture in light of the clinical performance must follow specific quality criteria, here discussed, which are relevant for the future use of any cultured cell type for clinical application.",
keywords = "Cell therapy, Cornea, Holoclone, Keratinocytes, Limbus, Regenerative medicine, Squamous epithelia, Tissue engineering",
author = "Francesca Corradini and Michela Zattoni and Paolo Rama and {De Luca}, Michele and Graziella Pellegrini",
year = "2014",
doi = "10.1016/B978-0-12-398523-1.00059-8",
language = "English",
isbn = "9780123985231",
pages = "829--840",
booktitle = "Regenerative Medicine Applications in Organ Transplantation",
publisher = "Elsevier Inc.",

}

TY - CHAP

T1 - Corneal Bioengineering

AU - Corradini, Francesca

AU - Zattoni, Michela

AU - Rama, Paolo

AU - De Luca, Michele

AU - Pellegrini, Graziella

PY - 2014

Y1 - 2014

N2 - The cornea is the main structure of the ocular surface and enables the transmission of light entering the eye. The cornea is covered by a nonkeratinized stratified epithelium, which is renewed by stem cells located in the basal layer of the limbus. Injury and disease, such as chemical and thermal burns, can destroy the limbus leading to a limbal stem cell deficiency (LSCD) with consequent visual loss. In 1997, Pellegrini et al. firstly described the use of ex vivo cultured limbal epithelial stem cell to treat LSCD. Since then, several reports describing alternative methods of the clinical use of this technology have been published but the retention of stem cells, which is the essential property of the graft, has not been investigated. The definition of a graftable limbal culture in light of the clinical performance must follow specific quality criteria, here discussed, which are relevant for the future use of any cultured cell type for clinical application.

AB - The cornea is the main structure of the ocular surface and enables the transmission of light entering the eye. The cornea is covered by a nonkeratinized stratified epithelium, which is renewed by stem cells located in the basal layer of the limbus. Injury and disease, such as chemical and thermal burns, can destroy the limbus leading to a limbal stem cell deficiency (LSCD) with consequent visual loss. In 1997, Pellegrini et al. firstly described the use of ex vivo cultured limbal epithelial stem cell to treat LSCD. Since then, several reports describing alternative methods of the clinical use of this technology have been published but the retention of stem cells, which is the essential property of the graft, has not been investigated. The definition of a graftable limbal culture in light of the clinical performance must follow specific quality criteria, here discussed, which are relevant for the future use of any cultured cell type for clinical application.

KW - Cell therapy

KW - Cornea

KW - Holoclone

KW - Keratinocytes

KW - Limbus

KW - Regenerative medicine

KW - Squamous epithelia

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=84902910991&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902910991&partnerID=8YFLogxK

U2 - 10.1016/B978-0-12-398523-1.00059-8

DO - 10.1016/B978-0-12-398523-1.00059-8

M3 - Chapter

SN - 9780123985231

SP - 829

EP - 840

BT - Regenerative Medicine Applications in Organ Transplantation

PB - Elsevier Inc.

ER -