Corneal involvement in graves' orbitopathy: An in vivo confocal study

Edoardo Villani, Francesco Viola, Roberto Sala, Mario Salvi, Chiara Mapelli, Nicola Currò, Guia Vannucchi, Paolo Beck-Peccoz, Roberto Ratiglia

Research output: Contribution to journalArticlepeer-review


To study the clinical involvement of the ocular surface and the in vivo morphology of corneal cells and nerves, in patients affected by active and inactive Graves' orbitopathy (GO). METHODS. The study included 26 consecutive GO patients and 20 age- and sex-matched healthy control subjects. GO was diagnosed on the basis of the criteria of the European Group on Graves' Orbitopathy, and disease activity was evaluated by the Clinical Activity Score (CAS). Each participant underwent a full eye examination, including an evaluation of symptoms (Ocular Surface Disease Index score), tear break-up time, fluorescein and lissamine green staining, corneal apex sensitivity, and Schirmer's test. The corneal apex was examined by means of confocal microscopy to investigate the number and morphology of epithelial and stromal corneal cells and subbasal nerves. RESULTS. Eleven (43%) of the 26 patients had active GO. Oneway ANOVA with the least-significant difference (LSD) post hoc test revealed statistically significant differences between patients and controls in all the evaluated parameters, except corneal sensitivity and nerve reflectivity. Among the GO patients, the only significant difference observed in active compared with inactive disease was in the number of hyperreflective (activated) keratocytes (P <0.001, LSD). Corneal sensitivity correlated inversely with proptosis (P <0.001, Spearman's test). CONCLUSIONS. GO patients show clinical and confocal corneal alterations and signs and symptoms partially related to dry eye disease. The ocular surface inflammation in GO seems to be due to both the dry eye and the autoimmune orbitopathy.

Original languageEnglish
Pages (from-to)4574-4578
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Issue number9
Publication statusPublished - Sep 2010

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)


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