Coronaviridae and SARS-associated Coronavirus Strain HSR1

Elisa Vicenzi, Filippo Canducci, Debora Pinna, Nicasio Mancini, Silvia Carletti, Adriano Lazzarin, Claudio Bordignon, Guido Poli, Massimo Clementi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

During the recent severe acute respiratory syndrome (SARS) outbreak, the etiologic agent was identified as a new coronavirus (CoV). We have isolated a SARS-associated CoV (SARS-CoV) strain by injecting Vero cells with a sputum specimen from an Italian patient affected by a severe pneumonia; the patient traveled from Vietnam to Italy in March 2003. Ultrastructural analysis of infected Vero cells showed the virions within cell vesicles and around the cell membrane. The full-length viral genome sequence was similar to those derived from the Hong-Kong Hotel M isolate. By using both real-time reverse transcription-polymerase chain reaction TaqMan assay and an infectivity plaque assay, we determined that approximately 360 viral genomes were required to generate a PFU. In addition, heparin (100 mg/mL) inhibited infection of Vero cells by 50%. Overall, the molecular and biologic characteristics of the strain HSR1 provide evidence that SARS-CoV forms a fourth genetic coronavirus group with distinct genomic and biologic features.

Original languageEnglish
Pages (from-to)413-418
Number of pages6
JournalEmerging Infectious Diseases
Volume10
Issue number3
Publication statusPublished - Mar 2004

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Coronaviridae
Severe Acute Respiratory Syndrome
Coronavirus
Vero Cells
Viral Genome
Vietnam
Hong Kong
Sputum
Virion
Italy
Reverse Transcription
Disease Outbreaks
Heparin
Pneumonia
Cell Membrane
Polymerase Chain Reaction
Infection

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Coronaviridae and SARS-associated Coronavirus Strain HSR1. / Vicenzi, Elisa; Canducci, Filippo; Pinna, Debora; Mancini, Nicasio; Carletti, Silvia; Lazzarin, Adriano; Bordignon, Claudio; Poli, Guido; Clementi, Massimo.

In: Emerging Infectious Diseases, Vol. 10, No. 3, 03.2004, p. 413-418.

Research output: Contribution to journalArticle

Vicenzi, E, Canducci, F, Pinna, D, Mancini, N, Carletti, S, Lazzarin, A, Bordignon, C, Poli, G & Clementi, M 2004, 'Coronaviridae and SARS-associated Coronavirus Strain HSR1', Emerging Infectious Diseases, vol. 10, no. 3, pp. 413-418.
Vicenzi E, Canducci F, Pinna D, Mancini N, Carletti S, Lazzarin A et al. Coronaviridae and SARS-associated Coronavirus Strain HSR1. Emerging Infectious Diseases. 2004 Mar;10(3):413-418.
Vicenzi, Elisa ; Canducci, Filippo ; Pinna, Debora ; Mancini, Nicasio ; Carletti, Silvia ; Lazzarin, Adriano ; Bordignon, Claudio ; Poli, Guido ; Clementi, Massimo. / Coronaviridae and SARS-associated Coronavirus Strain HSR1. In: Emerging Infectious Diseases. 2004 ; Vol. 10, No. 3. pp. 413-418.
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