Corrective transduction of human epidermal stem cells in laminin-5-dependent junctional epidermolysis bullosa

Elena Dellambra, Joëlle Vailly, Graziella Pellegrini, Sergio Bondanza, Osvaldo Golisano, Cinzia Macchia, Giovanna Zambruno, Guerrino Meneguzzi, Michele De Luca

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Laminin-5 is composed of three distinct polypeptides, α3, β3, and γ2, which are encoded by three different genes, LAMA3, LAMB3, and LAMC2, respectively. We have isolated epidermal keratinocytes from a patient presenting with a lethal form of junctional epidermolysis bullosa characterized by a homozygous mutation of the LAMB3 gene, which led to complete absence of the β3 polypeptide. In vitro, β3-null keratinocytes were unable to synthesize laminin-5 and to assemble hemidesmosomes, maintained the impairment of their adhesive properties, and displayed a decrease of their colony-forming ability. A retroviral construct expressing a human β3 cDNA was used to transduce primary β3-null keratinocytes. Clonogenic β3-null keratinocytes were transduced with an efficiency of 100%. β3-transduced keratinocytes were able to synthesize and secrete mature heterotrimeric laminin-5. Gene correction fully restored the keratinocyte adhesion machinery, including the capacity of proper hemidesmosomal assembly, and prevented the loss of the colony-forming ability, suggesting a direct link between adhesion to laminin-5 and keratinocyte proliferative capacity. Clonal analysis demonstrated that holoclones expressed the transgene permanently, suggesting stable correction of epidermal stem cells. Because cultured keratinocytes are used routinely to make autologous grafts for patients suffering from large skin or mucosal defects, the full phenotypic reversion of primary human epidermal stem cells defective for a structural protein opens new perspectives in the long-term treatment of genodermatoses.

Original languageEnglish
Pages (from-to)1359-1370
Number of pages12
JournalHuman Gene Therapy
Volume9
Issue number9
Publication statusPublished - Jun 10 1998

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Junctional Epidermolysis Bullosa
Keratinocytes
Stem Cells
Hemidesmosomes
Genes
Peptides
kalinin
Transgenes
Adhesives
Complementary DNA
Transplants

ASJC Scopus subject areas

  • Genetics

Cite this

Dellambra, E., Vailly, J., Pellegrini, G., Bondanza, S., Golisano, O., Macchia, C., ... De Luca, M. (1998). Corrective transduction of human epidermal stem cells in laminin-5-dependent junctional epidermolysis bullosa. Human Gene Therapy, 9(9), 1359-1370.

Corrective transduction of human epidermal stem cells in laminin-5-dependent junctional epidermolysis bullosa. / Dellambra, Elena; Vailly, Joëlle; Pellegrini, Graziella; Bondanza, Sergio; Golisano, Osvaldo; Macchia, Cinzia; Zambruno, Giovanna; Meneguzzi, Guerrino; De Luca, Michele.

In: Human Gene Therapy, Vol. 9, No. 9, 10.06.1998, p. 1359-1370.

Research output: Contribution to journalArticle

Dellambra, E, Vailly, J, Pellegrini, G, Bondanza, S, Golisano, O, Macchia, C, Zambruno, G, Meneguzzi, G & De Luca, M 1998, 'Corrective transduction of human epidermal stem cells in laminin-5-dependent junctional epidermolysis bullosa', Human Gene Therapy, vol. 9, no. 9, pp. 1359-1370.
Dellambra E, Vailly J, Pellegrini G, Bondanza S, Golisano O, Macchia C et al. Corrective transduction of human epidermal stem cells in laminin-5-dependent junctional epidermolysis bullosa. Human Gene Therapy. 1998 Jun 10;9(9):1359-1370.
Dellambra, Elena ; Vailly, Joëlle ; Pellegrini, Graziella ; Bondanza, Sergio ; Golisano, Osvaldo ; Macchia, Cinzia ; Zambruno, Giovanna ; Meneguzzi, Guerrino ; De Luca, Michele. / Corrective transduction of human epidermal stem cells in laminin-5-dependent junctional epidermolysis bullosa. In: Human Gene Therapy. 1998 ; Vol. 9, No. 9. pp. 1359-1370.
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