Correlation between folate and vitamin B12 and markers of DNA stability in healthy men: Preliminary results

Mirta Milić, Ružica Rozgaj, Vilena Kašuba, Višnja Oreščanin, Melita Balija, Irena Jukić

Research output: Contribution to journalArticle

Abstract

The aim of this study was to find correlations between folate and vitamin B12 on baseline damage in white blood cells and their association with smoking, alcohol consumption and ageing. Thirty-six healthy vitamin nondeficient male subjects were selected in a randomized study. Comet assay (SCGE) and micronucleus (MN) assay were used as biomarkers of DNA damage. The amount of DNA damage was correlated with vitamin B12 and folic acid concentration. Positive, but non-significant correlation (canonical R = 0.61; χ2 = 28.97; P = 0.253) was found between micronucleus (MN) frequency or comet assay parameters (SCGE) and five covariates (age, smoking, alcohol consumption, vitamin B12 and folate blood serum concentration). The highest MN frequency was observed in the group with the lowest vitamin B12 concentration (F = 3.59; P = 0.024). The SCGE assay failed to show significant correlation with vitamin B12 or folic acid concentration. Concentration of vitamin B12 was significantly correlated with incidence of micronuclei. Our results present background data that could be valuable for future genotoxicological monitoring.

Original languageEnglish
Pages (from-to)339-345
Number of pages7
JournalActa Biochimica Polonica
Volume57
Issue number3
Publication statusPublished - 2010

Fingerprint

Vitamin B 12
Genetic Markers
Folic Acid
Assays
DNA
Micronucleus Tests
Comet Assay
Alcohol Drinking
DNA Damage
Blood
Smoking
Alcohols
Biomarkers
Vitamins
Leukocytes
Aging of materials
Cells
Association reactions
Monitoring
Incidence

Keywords

  • Comet assay
  • DNA damage
  • Folic acid
  • Healthy men
  • Micronucleus
  • Vitamin B

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Milić, M., Rozgaj, R., Kašuba, V., Oreščanin, V., Balija, M., & Jukić, I. (2010). Correlation between folate and vitamin B12 and markers of DNA stability in healthy men: Preliminary results. Acta Biochimica Polonica, 57(3), 339-345.

Correlation between folate and vitamin B12 and markers of DNA stability in healthy men : Preliminary results. / Milić, Mirta; Rozgaj, Ružica; Kašuba, Vilena; Oreščanin, Višnja; Balija, Melita; Jukić, Irena.

In: Acta Biochimica Polonica, Vol. 57, No. 3, 2010, p. 339-345.

Research output: Contribution to journalArticle

Milić, M, Rozgaj, R, Kašuba, V, Oreščanin, V, Balija, M & Jukić, I 2010, 'Correlation between folate and vitamin B12 and markers of DNA stability in healthy men: Preliminary results', Acta Biochimica Polonica, vol. 57, no. 3, pp. 339-345.
Milić M, Rozgaj R, Kašuba V, Oreščanin V, Balija M, Jukić I. Correlation between folate and vitamin B12 and markers of DNA stability in healthy men: Preliminary results. Acta Biochimica Polonica. 2010;57(3):339-345.
Milić, Mirta ; Rozgaj, Ružica ; Kašuba, Vilena ; Oreščanin, Višnja ; Balija, Melita ; Jukić, Irena. / Correlation between folate and vitamin B12 and markers of DNA stability in healthy men : Preliminary results. In: Acta Biochimica Polonica. 2010 ; Vol. 57, No. 3. pp. 339-345.
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