Correlation between nitric oxide synthase activity and reduced glutathione level in human and murine endothelial cells

D. Ghigo, D. Geromin, C. Franchino, R. Todde, C. Priotto, C. Costamagna, M. Arese, G. Garbarino, G. P. Pescarmona, A. Bosia

Research output: Contribution to journalArticle

Abstract

The synthesis of nitric oxide (NO), detected as citrulline production, in human (HUVEC) and murine (tEnd.1) endothelial cells correlated with intracellular GSH. tEnd.1, which exhibited an intracellular GSH level 2.5-fold higher than HUVEC, showed a citrulline production (basally and after ionomycin stimulation) 5-8 times higher than human cells. Ionomycin-elicited citrulline synthesis in tEnd.1 cells increased 2.4-fold after loading with GSH, and decreased dose-dependently after GSH depletion. Cell loading with N-(2-mercaptopropionyl)-glycine neither significantly increased citrulline production nor relieved the effect of GSH depletion.

Original languageEnglish
Pages (from-to)277-281
Number of pages5
JournalAmino Acids
Volume10
Issue number3
DOIs
Publication statusPublished - 1996

Keywords

  • 1-chloro-2,4-dinitrobenzene
  • Amino acids
  • Endothelial cells
  • Glutathione
  • N-(2-mercaptopropionyl)-glycine
  • Nitric oxide
  • Polyomavirus

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry
  • Endocrinology

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    Ghigo, D., Geromin, D., Franchino, C., Todde, R., Priotto, C., Costamagna, C., Arese, M., Garbarino, G., Pescarmona, G. P., & Bosia, A. (1996). Correlation between nitric oxide synthase activity and reduced glutathione level in human and murine endothelial cells. Amino Acids, 10(3), 277-281. https://doi.org/10.1007/BF00807330