Correlation of der p 2 T-cell responses with clinical characteristics of children allergic to house dust mite

Valentina Pacciani, Stefania Corrente, Silvia Gregori, Andrea Pierantozzi, Romina Silenzi, Marco Chianca, Viviana Moschese, Loredana Chini, Federica Angelini

Research output: Contribution to journalArticlepeer-review

Abstract

Background: An understanding of the mechanisms responsible for the development and maintenance of allergic inflammation and their clinical implications is needed to develop specific and successful treatment for allergy. Objectives: To characterize in vitro T-cell responses to Der p 2, one of the major allergens of house dust mite (HDM), and investigate potential correlations between clinical and laboratory parameters. Methods: Forty-two patients monosensitized to HDM and 10 age-matched, healthy children were studied. Dendritic cells pulsed with Der p 2 were used to stimulate autologous CD14 - cells. Der p 2-specific T-cell activation markers, proliferation, and cytokine production profiles were examined. Results: Der p 2-specific T-cell activation markers, proliferation, and TH2 cytokine production were significantly higher in HDM patients compared with healthy controls. Moreover, a significant correlation between proliferation and TH2 cytokine production was observed. Within the allergic group, skin reaction to HDM was significantly stronger in patients with a Der p 2-specific T-cell response. Levels of HDM-specific IgE directly correlated with interleukin 5 and interleukin 13 levels and with skin prick test results and, ultimately, with the patient's family history of allergy. Furthermore, the presence of atopic march correlated with T-cell proliferation. Conclusion: We found that, in HDM patients, Der p 2-specific TH2 responses, promoted by autologous dendritic cells in vitro, correlate with clinical parameters.

Original languageEnglish
Pages (from-to)442-447
Number of pages6
JournalAnnals of Allergy, Asthma and Immunology
Volume109
Issue number6
DOIs
Publication statusPublished - Dec 2012

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pulmonary and Respiratory Medicine

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