Correlative light-electron microscopy reveals the tubular-saccular ultrastructure of carriers operating between Golgi apparatus and plasma membrane

Roman S. Polishchuk, Elena V. Polishchuk, Pierfrancesco Marra, Saverio Alberti, Roberto Buccione, Alberto Luini, Alexander A. Mironov

Research output: Contribution to journalArticle

Abstract

Transport intermediates (TIs) have a central role in intracellular traffic, and much effort has been directed towards defining their molecular organization. Unfortunately, major uncertainties remain regarding their true structure in living cells. To address this question, we have developed an approach based on the combination of the green fluorescent protein technology and correlative light-electron microscopy, by which it is possible to monitor an individual carrier in vivo and then take a picture of its ultrastructure at any moment of its lifecycle. We have applied this technique to define the structure of TIs operating from the Golgi apparatus to the plasma membrane, whose in vivo dynamics have been characterized recently by light microscopy. We find that these carriers are large (ranging from 0.3-1.7 μm in maximum diameter, nearly half the size of a Golgi cisterna), comprise almost exclusively tubularsaccular structures, and fuse directly with the plasma membrane, sometimes minutes after docking to the fusion site.

Original languageEnglish
Pages (from-to)45-58
Number of pages14
JournalJournal of Cell Biology
Volume148
Issue number1
DOIs
Publication statusPublished - Jan 10 2000

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Golgi Apparatus
Electron Microscopy
Cell Membrane
Light
Green Fluorescent Proteins
Uncertainty
Microscopy
Technology

Keywords

  • Anterograde intracellular transport
  • Golgi complex
  • Immunoelectron microscopy
  • Membrane traffic
  • Plasmalemma

ASJC Scopus subject areas

  • Cell Biology

Cite this

Correlative light-electron microscopy reveals the tubular-saccular ultrastructure of carriers operating between Golgi apparatus and plasma membrane. / Polishchuk, Roman S.; Polishchuk, Elena V.; Marra, Pierfrancesco; Alberti, Saverio; Buccione, Roberto; Luini, Alberto; Mironov, Alexander A.

In: Journal of Cell Biology, Vol. 148, No. 1, 10.01.2000, p. 45-58.

Research output: Contribution to journalArticle

Polishchuk, Roman S. ; Polishchuk, Elena V. ; Marra, Pierfrancesco ; Alberti, Saverio ; Buccione, Roberto ; Luini, Alberto ; Mironov, Alexander A. / Correlative light-electron microscopy reveals the tubular-saccular ultrastructure of carriers operating between Golgi apparatus and plasma membrane. In: Journal of Cell Biology. 2000 ; Vol. 148, No. 1. pp. 45-58.
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AU - Luini, Alberto

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