Cortical expression of brain derived neurotrophic factor and type-1 cannabinoid receptor after striatal excitotoxic lesions

Z. De March, C. Zuccato, C. Giampà, S. Patassini, M. Bari, V. Gasperi, M. L. De Ceballos, G. Bernardi, M. Maccarrone, E. Cattaneo, F. R. Fusco

Research output: Contribution to journalArticle

Abstract

An involvement of one particular neurotrophin, namely, the brain-derived neurotrophic factor (BDNF), has been demonstrated in the pathophysiology Huntington's disease. Type-1 cannabinoid (CB1) receptor has been postulated to upregulate BDNF gene transcription. To better understand the relationship between CB1 and BDNF levels in a situation where the striatum is degenerating, we studied, by dual label immunofluorescence, the distribution of CB1 and BDNF in cortical neurons projecting to the striatum in our rat quinolinic acid model of striatal excitotoxicity. We completed our study with quantitative analyses of BDNF protein levels and CB1 binding activity in the cortex. We show that, 2 weeks post lesion, cortical neurons contain more BDNF compared with controls and to earlier time points. Such BDNF up-regulation coincides with a higher binding activity and an increased protein expression of CB1. We suggest that after excitotoxic lesions, CB1 might, at least transiently, upregulate BDNF in the attempt to rescue striatal neurons from degeneration.

Original languageEnglish
Pages (from-to)734-740
Number of pages7
JournalNeuroscience
Volume152
Issue number3
DOIs
Publication statusPublished - Mar 27 2008

Fingerprint

Corpus Striatum
Cannabinoid Receptors
Brain-Derived Neurotrophic Factor
Up-Regulation
Nerve Growth Factors
Quinolinic Acid
Neurons
Cannabinoid Receptor CB1
Nerve Degeneration
Huntington Disease
Fluorescent Antibody Technique

Keywords

  • BDNF
  • CB1
  • confocal microscopy
  • Huntington's disease
  • quinolinic acid
  • rat

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cortical expression of brain derived neurotrophic factor and type-1 cannabinoid receptor after striatal excitotoxic lesions. / De March, Z.; Zuccato, C.; Giampà, C.; Patassini, S.; Bari, M.; Gasperi, V.; De Ceballos, M. L.; Bernardi, G.; Maccarrone, M.; Cattaneo, E.; Fusco, F. R.

In: Neuroscience, Vol. 152, No. 3, 27.03.2008, p. 734-740.

Research output: Contribution to journalArticle

De March, Z, Zuccato, C, Giampà, C, Patassini, S, Bari, M, Gasperi, V, De Ceballos, ML, Bernardi, G, Maccarrone, M, Cattaneo, E & Fusco, FR 2008, 'Cortical expression of brain derived neurotrophic factor and type-1 cannabinoid receptor after striatal excitotoxic lesions', Neuroscience, vol. 152, no. 3, pp. 734-740. https://doi.org/10.1016/j.neuroscience.2007.11.044
De March, Z. ; Zuccato, C. ; Giampà, C. ; Patassini, S. ; Bari, M. ; Gasperi, V. ; De Ceballos, M. L. ; Bernardi, G. ; Maccarrone, M. ; Cattaneo, E. ; Fusco, F. R. / Cortical expression of brain derived neurotrophic factor and type-1 cannabinoid receptor after striatal excitotoxic lesions. In: Neuroscience. 2008 ; Vol. 152, No. 3. pp. 734-740.
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