Cortical fibroblast culture from human biopsies

F. Strutz, A. Renziehausen, M. Dietrich, J. Amin, V. Becker, M. Heeg, M. P. Rastaldi, G. A. Müller

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Tubulointerstitial fibrosis is an integral part of progressive renal disease. Human cortical fibroblasts are believed to be key effector cells in fibrogenesis. Thus, a reliable culture of these cells is necessary for studies of their pathophysiology. Methods: Cortical fibroblast culture from routine kidney biopsies were analyzed and the cells were characterized. Indirect immunofluorescence staining was done after the first passage for cytokeratin, vimentin, α-smooth muscle actin, CD 44, CD 54, CD 68, collagen types I, III, and HLA-DR. We then assessed the utility of the putative fibroblast markers CD 90, prolyl-4-hydroxylase (P4H) and F1b in simultaneous stainings of tubular epithelial cells. Results: During the study period, 49 biopsy cores were cultured and cortical fibroblasts could be successfully established in 21 cases (42.9%). There was no relation between the success rate of culture and the degree of interstitial fibrosis, but an association was seen with the time of completion of the first passage. There was a negative correlation between the extent of scarring and the percentage of cytokeratin positive cells (r=-0.66, p

Original languageEnglish
Pages (from-to)190-197
Number of pages8
JournalJournal of Nephrology
Volume14
Issue number3
Publication statusPublished - 2001

Keywords

  • α-smooth muscle actin
  • CD 44
  • CD 54
  • CD 90
  • Extracellular matrix
  • Kidney fibrosis

ASJC Scopus subject areas

  • Nephrology

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