TY - JOUR
T1 - Corticocancellous porcine bone in the healing of human extraction sockets
T2 - combining histomorphometry with osteoblast gene expression profiles in vivo.
AU - Crespi, Roberto
AU - Capparé, Paolo
AU - Romanos, Georgios E.
AU - Mariani, Elisabetta
AU - Benasciutti, Elisa
AU - Gherlone, Enrico
PY - 2011/7
Y1 - 2011/7
N2 - Different graft materials have been proposed to minimize the collapse of alveolar bone after tooth extraction. The aim of this study was to examine the use of porcine bone graft in fresh sockets via histomorphometric and in vivo gene expression profiling. Thirty fresh extraction sockets with three bone walls in 15 patients were selected. A split-mouth design was employed. On one side of the arch, 15 sockets received corticocancellous porcine bone as a graft, and on the other side, 15 sockets were left unfilled and considered as controls. Four months after surgery, four biopsy specimens were taken from each patient (two from the grafted site and two from the control site). The specimens were analyzed by histomorphometry and ex vivo osteoblast expansion, followed by highly sensitive osteoblast-specific gene expression profiling for Runx2, osteopontin, osteoprotegerin, type I collagen, and alkaline phosphatase by quantitative real-time reverse-transcriptase polymerase chain reaction. Comparisons were made using the Student t test. After healing without complications, the grafted sites showed statistically significantly higher mean vital bone and lower mean connective tissue values than the control sites. Statistically significant higher expression of alkaline phosphatase and the matrix formation markers type I collagen and osteopontin were observed in the grafted group compared to the control group, whereas Runx2 and osteoprotegerin expression was comparable. Within the limits of this study, histologic examination and biomolecular evaluation confirmed good biocompatibility and high osteoconductivity of xenogeneic porcine bone in alveolar bone grafting.
AB - Different graft materials have been proposed to minimize the collapse of alveolar bone after tooth extraction. The aim of this study was to examine the use of porcine bone graft in fresh sockets via histomorphometric and in vivo gene expression profiling. Thirty fresh extraction sockets with three bone walls in 15 patients were selected. A split-mouth design was employed. On one side of the arch, 15 sockets received corticocancellous porcine bone as a graft, and on the other side, 15 sockets were left unfilled and considered as controls. Four months after surgery, four biopsy specimens were taken from each patient (two from the grafted site and two from the control site). The specimens were analyzed by histomorphometry and ex vivo osteoblast expansion, followed by highly sensitive osteoblast-specific gene expression profiling for Runx2, osteopontin, osteoprotegerin, type I collagen, and alkaline phosphatase by quantitative real-time reverse-transcriptase polymerase chain reaction. Comparisons were made using the Student t test. After healing without complications, the grafted sites showed statistically significantly higher mean vital bone and lower mean connective tissue values than the control sites. Statistically significant higher expression of alkaline phosphatase and the matrix formation markers type I collagen and osteopontin were observed in the grafted group compared to the control group, whereas Runx2 and osteoprotegerin expression was comparable. Within the limits of this study, histologic examination and biomolecular evaluation confirmed good biocompatibility and high osteoconductivity of xenogeneic porcine bone in alveolar bone grafting.
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M3 - Article
C2 - 21841997
AN - SCOPUS:84555208807
VL - 26
SP - 866
EP - 872
JO - International Journal of Oral and Maxillofacial Implants
JF - International Journal of Oral and Maxillofacial Implants
SN - 0882-2786
IS - 4
ER -