Corticosteroids regulate the gene expression of FGF-1 and FGF-2 in cultured rat astrocytes

V. Magnaghi, M. A. Riva, I. Cavarretta, L. Martini, R. C. Melcangi

Research output: Contribution to journalArticle

Abstract

The present data show that the gene expression of FGF-1 and FGF-2 is regulated by corticosteroids in rat type 1 astrocytes. In particular, the gene expression of FGF-1 is modulated by corticosteroids acting both on type I (minerocorticoid) and type II (glucocorticoid) receptors. In fact, at short times of exposure (2 h) a slight decrease in FGF-1 mRNA levels is induced by deoxycorticosterone, a steroid able to interact with the type I receptors; a similar effect is observed at 6 h following exposure to corticosterone or its 5α-reduced metabolite, dihydrocorticosterone. Conversely, at longer times of exposure (24 h) corticosterone is able to strongly increase FGF-1 mRNA levels. Both effects of corticosterone (inhibition and stimulation) were duplicated by dexamethasone, indicating that both effects occur via the type II receptors. Interestingly, the 5α-3α-reduced metabolite of deoxycorticosterone, tetrahydrodeoxycorticosterone, which does not interact with either corticosteroid receptors, is able to stimulate (at 6 and 24 h of exposure) the gene expression of FGF-1. It is possible that this effect might be induced via the GABAA receptor, since muscimol, an agonist of this receptor, exerts a similar effect. The situation is different in the case of FGF-2. The mRNA levels of this growth factor are only stimulated by steroids interacting with type II receptors. Altogether, these observations indicate that corticosteroids modulate the levels of FGF-1 and FGF-2 gene expression in astroglial cells by interaction with classical (type I and II) or nonclassical (GABAA receptor) steroid receptors.

Original languageEnglish
Pages (from-to)11-18
Number of pages8
JournalJournal of Molecular Neuroscience
Volume15
Issue number1
DOIs
Publication statusPublished - 2000

Keywords

  • 5α- and 5α-3α-reduced metabolites
  • Astrocytes
  • Corticosterone
  • FGF-1
  • FGF-2
  • GABA receptor
  • Type I and II corticosteroid receptors

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry
  • Genetics

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