Corticotropin releasing factor stimulates cAMP formation in pituitary corticotropic tumor cells

Marco Parenti, Luigi Cantalamessa, Anna Catania, Eugenio Reschini, Eugenio E. Müller

Research output: Contribution to journalArticlepeer-review

Abstract

Addition of corticotropin-releasing factor (CRF) to membranes from two ACTH-secreting pituitary tumors strikingly increased in a dose-dependent fashion adenylate cyclase (AC) activity. Significant stimulation was already apparent at 10-9M CRF. Stimulation of AC activity by CRF in membranes from non-tumoral tissue adjacent to tumoral corticotrophs was considerably lower, and was lacking in membranes from a growth hormone secreting tumor. These data correlated well with in vivo pre-surgery and post-surgery ACTH reponsiveness to CRF of the tumor bearing patients. Basal AC activity was higher in pituitary adenomas than in non-tumoral adjacent tissue. It is concluded that 1) a CRF-sensitive AC exists in ACTH-secreting tumor cells and, 2) increased sensitivity to CRF, as evidenced by greater stimulation of AC activity, may be responsible for the increased ACTH output of tumoral corticotrophs.

Original languageEnglish
Pages (from-to)359-363
Number of pages5
JournalLife Sciences
Volume34
Issue number4
DOIs
Publication statusPublished - Jan 23 1984

ASJC Scopus subject areas

  • Pharmacology

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