Abstract
Addition of corticotropin-releasing factor (CRF) to membranes from two ACTH-secreting pituitary tumors strikingly increased in a dose-dependent fashion adenylate cyclase (AC) activity. Significant stimulation was already apparent at 10-9M CRF. Stimulation of AC activity by CRF in membranes from non-tumoral tissue adjacent to tumoral corticotrophs was considerably lower, and was lacking in membranes from a growth hormone secreting tumor. These data correlated well with in vivo pre-surgery and post-surgery ACTH reponsiveness to CRF of the tumor bearing patients. Basal AC activity was higher in pituitary adenomas than in non-tumoral adjacent tissue. It is concluded that 1) a CRF-sensitive AC exists in ACTH-secreting tumor cells and, 2) increased sensitivity to CRF, as evidenced by greater stimulation of AC activity, may be responsible for the increased ACTH output of tumoral corticotrophs.
| Original language | English |
|---|---|
| Pages (from-to) | 359-363 |
| Number of pages | 5 |
| Journal | Life Sciences |
| Volume | 34 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Jan 23 1984 |
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ASJC Scopus subject areas
- Pharmacology
Cite this
Corticotropin releasing factor stimulates cAMP formation in pituitary corticotropic tumor cells. / Parenti, Marco; Cantalamessa, Luigi; Catania, Anna; Reschini, Eugenio; Müller, Eugenio E.
In: Life Sciences, Vol. 34, No. 4, 23.01.1984, p. 359-363.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Corticotropin releasing factor stimulates cAMP formation in pituitary corticotropic tumor cells
AU - Parenti, Marco
AU - Cantalamessa, Luigi
AU - Catania, Anna
AU - Reschini, Eugenio
AU - Müller, Eugenio E.
PY - 1984/1/23
Y1 - 1984/1/23
N2 - Addition of corticotropin-releasing factor (CRF) to membranes from two ACTH-secreting pituitary tumors strikingly increased in a dose-dependent fashion adenylate cyclase (AC) activity. Significant stimulation was already apparent at 10-9M CRF. Stimulation of AC activity by CRF in membranes from non-tumoral tissue adjacent to tumoral corticotrophs was considerably lower, and was lacking in membranes from a growth hormone secreting tumor. These data correlated well with in vivo pre-surgery and post-surgery ACTH reponsiveness to CRF of the tumor bearing patients. Basal AC activity was higher in pituitary adenomas than in non-tumoral adjacent tissue. It is concluded that 1) a CRF-sensitive AC exists in ACTH-secreting tumor cells and, 2) increased sensitivity to CRF, as evidenced by greater stimulation of AC activity, may be responsible for the increased ACTH output of tumoral corticotrophs.
AB - Addition of corticotropin-releasing factor (CRF) to membranes from two ACTH-secreting pituitary tumors strikingly increased in a dose-dependent fashion adenylate cyclase (AC) activity. Significant stimulation was already apparent at 10-9M CRF. Stimulation of AC activity by CRF in membranes from non-tumoral tissue adjacent to tumoral corticotrophs was considerably lower, and was lacking in membranes from a growth hormone secreting tumor. These data correlated well with in vivo pre-surgery and post-surgery ACTH reponsiveness to CRF of the tumor bearing patients. Basal AC activity was higher in pituitary adenomas than in non-tumoral adjacent tissue. It is concluded that 1) a CRF-sensitive AC exists in ACTH-secreting tumor cells and, 2) increased sensitivity to CRF, as evidenced by greater stimulation of AC activity, may be responsible for the increased ACTH output of tumoral corticotrophs.
UR - http://www.scopus.com/inward/record.url?scp=0021358488&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021358488&partnerID=8YFLogxK
U2 - 10.1016/0024-3205(84)90624-6
DO - 10.1016/0024-3205(84)90624-6
M3 - Article
C2 - 6319941
AN - SCOPUS:0021358488
VL - 34
SP - 359
EP - 363
JO - Life Sciences
JF - Life Sciences
SN - 0024-3205
IS - 4
ER -