Corticotropin-releasing hormone modulates cytokines release in cultured human peripheral blood mononuclear cells

Stefano Angioni, Felice Petraglia, Andrea Gallinelli, Andrea Cossarizza, Claudio Franceschi, Michela Muscettola, Alessandro D. Genazzani, Nicola Surico, Andrea R. Genazzani

Research output: Contribution to journalArticle

Abstract

Immune and neuroendocrine systems interact at various levels. In particular, either cytokines activate the hypothalamus-pituitary-adrenal axis (HPA) or corticotropin-releasing hormone (CRH) induces the release of β-endorphin from peripheral human mononuclear cells. The aim of the present study was to investigate whether CRH may affect cytokine production and activity in human peripheral blood mononuclear cells (PBMC). Primary cultures of human PBMC and monocytes were used. They were incubated in presence of different doses of synthetic human CRH. Media were collected and interleukin-1β (IL-1β) and interleukin-6 (IL-6) levels were measured by ELISA, while interferon-gamma (IFN-γ) levels were measured by bioassay. In addition, phytohemoagglutinin-induced lymphocyte proliferation was evaluated by testing [3H]thymidine incorporation in the presence of various doses of CRH. CRH significantly increased IL-6 release from PBMC (p

Original languageEnglish
Pages (from-to)1735-1742
Number of pages8
JournalLife Sciences
Volume53
Issue number23
DOIs
Publication statusPublished - 1993

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ASJC Scopus subject areas

  • Pharmacology

Cite this

Angioni, S., Petraglia, F., Gallinelli, A., Cossarizza, A., Franceschi, C., Muscettola, M., Genazzani, A. D., Surico, N., & Genazzani, A. R. (1993). Corticotropin-releasing hormone modulates cytokines release in cultured human peripheral blood mononuclear cells. Life Sciences, 53(23), 1735-1742. https://doi.org/10.1016/0024-3205(93)90160-5