Could the low level of expression of the gene encoding skeletal muscle mitofusin-2 account for the metabolic inflexibility of obesity?

G. Mingrone, M. Manco, M. Calvani, M. Castagneto, D. Naon, A. Zorzano

Research output: Contribution to journalArticle

Abstract

Aims/hypothesis: In obesity the cellular capacity to switch from using lipid to carbohydrate and vice versa as the energy substrate, known as 'metabolic flexibility', is impaired. Mitofusin 2 (MFN2), a mitochondrial membrane protein, seems to contribute to the maintenance and operation of the mitochondrial network, and its expression is reduced in obesity. The aim of this study was to verify whether MFN2 might be implicated in the metabolic inflexibility of obesity. Materials andmethods: Insulin sensitivity was measured in six morbidly obese women before and 2 years after malabsorptive bariatric surgery (BMI 53.3±10.5 vs 30.3±4.0 kg/m2). Skeletal muscle MFN2, SLC2A4 (formerly known as GLUT4), COX3 (encoding cytochrome c oxidase subunit III) and CS (encoding citrate synthase) mRNA levels were measured by real-time PCR. Results: Following bilio-pancreatic surgery, significant increases in MFN2 mRNA (from 0.4±0.2 to 1.7±1.1 arbitrary units [AU], p=0.019) and SLC2A4 mRNA (0.38±0.12 to 0.76±0.24 AU, p=0.04) were observed, while increases in COX3 mRNA (from 14.2±6.4 to 20.2±12.5 AU) and CS mRNA (from 0.4±0.1 to 0.7±0.3 AU) failed to reach statistical significance. Insulin-mediated whole-body glucose uptake significantly (p-1 min-1 and glucose oxidation rose from 11.1±2.1 to 37.7±4.7 μmol kg fat-free mass-1 min-1 (p2=0.92, p2=0.80, p2=0.61, p

Original languageEnglish
Pages (from-to)2108-2114
Number of pages7
JournalDiabetologia
Volume48
Issue number10
DOIs
Publication statusPublished - Oct 2005

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Keywords

  • Glucose oxidation
  • Insulin sensitivity
  • Metabolic inflexibility
  • Mitofusin-2 mRNA expression
  • Morbid obesity

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

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