Counterflow centrifugal elutriation: Present and future

C. Almici, C. Carlo-Stella, A. D. Donnenberg, V. Rizzoli

Research output: Contribution to journalArticle

Abstract

Counterflow centrifugal elutriation (CCE) has been proposed as a method for separating heterogeneous cell populations into distinct subpopulations on the basis of different sedimentation characteristics, without impairment of cell function or yield. The advantages of this technique are the high recovery and viability of fractionated cells and the rapidity and reproducibility of results. CCE alone or in combination with other separation methods can provide homogeneous populations of cells for further investigations. Recently, CCE has been employed in clinical studies aimed at preventing GVHD in BMT recipients by depleting lymphocytes prior to BM infusion. Furthermore, ongoing studies are concentrating on the use of negative selection procedures on the fractions currently excluded from the graft with the purpose of adding these depleted fractions to the graft preparation to augment the number of stem cells, accessory cells and unselected lymphocyte subsets. In the experimental field, CCE combined with negative and positive selection techniques may be useful in the study of hematopoiesis by separating 'pure' stem cell populations from more committed hematopoietic progenitors. We review here the present and possible future applications of elutriation in the clinical and experimental field.

Original languageEnglish
Pages (from-to)105-108
Number of pages4
JournalBone Marrow Transplantation
Volume12
Issue number2
Publication statusPublished - 1993

Fingerprint

Stem Cells
Population
Transplants
Lymphocyte Subsets
Hematopoiesis
Reproducibility of Results
Cell Survival
Lymphocytes
Clinical Studies

ASJC Scopus subject areas

  • Hematology
  • Transplantation

Cite this

Almici, C., Carlo-Stella, C., Donnenberg, A. D., & Rizzoli, V. (1993). Counterflow centrifugal elutriation: Present and future. Bone Marrow Transplantation, 12(2), 105-108.

Counterflow centrifugal elutriation : Present and future. / Almici, C.; Carlo-Stella, C.; Donnenberg, A. D.; Rizzoli, V.

In: Bone Marrow Transplantation, Vol. 12, No. 2, 1993, p. 105-108.

Research output: Contribution to journalArticle

Almici, C, Carlo-Stella, C, Donnenberg, AD & Rizzoli, V 1993, 'Counterflow centrifugal elutriation: Present and future', Bone Marrow Transplantation, vol. 12, no. 2, pp. 105-108.
Almici, C. ; Carlo-Stella, C. ; Donnenberg, A. D. ; Rizzoli, V. / Counterflow centrifugal elutriation : Present and future. In: Bone Marrow Transplantation. 1993 ; Vol. 12, No. 2. pp. 105-108.
@article{edad299e33564a1c98566cc4d9699de1,
title = "Counterflow centrifugal elutriation: Present and future",
abstract = "Counterflow centrifugal elutriation (CCE) has been proposed as a method for separating heterogeneous cell populations into distinct subpopulations on the basis of different sedimentation characteristics, without impairment of cell function or yield. The advantages of this technique are the high recovery and viability of fractionated cells and the rapidity and reproducibility of results. CCE alone or in combination with other separation methods can provide homogeneous populations of cells for further investigations. Recently, CCE has been employed in clinical studies aimed at preventing GVHD in BMT recipients by depleting lymphocytes prior to BM infusion. Furthermore, ongoing studies are concentrating on the use of negative selection procedures on the fractions currently excluded from the graft with the purpose of adding these depleted fractions to the graft preparation to augment the number of stem cells, accessory cells and unselected lymphocyte subsets. In the experimental field, CCE combined with negative and positive selection techniques may be useful in the study of hematopoiesis by separating 'pure' stem cell populations from more committed hematopoietic progenitors. We review here the present and possible future applications of elutriation in the clinical and experimental field.",
author = "C. Almici and C. Carlo-Stella and Donnenberg, {A. D.} and V. Rizzoli",
year = "1993",
language = "English",
volume = "12",
pages = "105--108",
journal = "Bone Marrow Transplantation",
issn = "0268-3369",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Counterflow centrifugal elutriation

T2 - Present and future

AU - Almici, C.

AU - Carlo-Stella, C.

AU - Donnenberg, A. D.

AU - Rizzoli, V.

PY - 1993

Y1 - 1993

N2 - Counterflow centrifugal elutriation (CCE) has been proposed as a method for separating heterogeneous cell populations into distinct subpopulations on the basis of different sedimentation characteristics, without impairment of cell function or yield. The advantages of this technique are the high recovery and viability of fractionated cells and the rapidity and reproducibility of results. CCE alone or in combination with other separation methods can provide homogeneous populations of cells for further investigations. Recently, CCE has been employed in clinical studies aimed at preventing GVHD in BMT recipients by depleting lymphocytes prior to BM infusion. Furthermore, ongoing studies are concentrating on the use of negative selection procedures on the fractions currently excluded from the graft with the purpose of adding these depleted fractions to the graft preparation to augment the number of stem cells, accessory cells and unselected lymphocyte subsets. In the experimental field, CCE combined with negative and positive selection techniques may be useful in the study of hematopoiesis by separating 'pure' stem cell populations from more committed hematopoietic progenitors. We review here the present and possible future applications of elutriation in the clinical and experimental field.

AB - Counterflow centrifugal elutriation (CCE) has been proposed as a method for separating heterogeneous cell populations into distinct subpopulations on the basis of different sedimentation characteristics, without impairment of cell function or yield. The advantages of this technique are the high recovery and viability of fractionated cells and the rapidity and reproducibility of results. CCE alone or in combination with other separation methods can provide homogeneous populations of cells for further investigations. Recently, CCE has been employed in clinical studies aimed at preventing GVHD in BMT recipients by depleting lymphocytes prior to BM infusion. Furthermore, ongoing studies are concentrating on the use of negative selection procedures on the fractions currently excluded from the graft with the purpose of adding these depleted fractions to the graft preparation to augment the number of stem cells, accessory cells and unselected lymphocyte subsets. In the experimental field, CCE combined with negative and positive selection techniques may be useful in the study of hematopoiesis by separating 'pure' stem cell populations from more committed hematopoietic progenitors. We review here the present and possible future applications of elutriation in the clinical and experimental field.

UR - http://www.scopus.com/inward/record.url?scp=0027160651&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027160651&partnerID=8YFLogxK

M3 - Article

C2 - 8401353

AN - SCOPUS:0027160651

VL - 12

SP - 105

EP - 108

JO - Bone Marrow Transplantation

JF - Bone Marrow Transplantation

SN - 0268-3369

IS - 2

ER -