Counterimmunoelectrophoresis, ELISA and immunoblotting detection of anti-Ro/SSA antibodies in subacute cutaneous lupus erythematosus. A comparative study

A. Parodi, M. Drosera, L. Barbieri, A. Rebora

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Abstract

Patients with subacute cutaneous lupus erythematosus (SCLE) have circulating antibodies to Ro/SSA directed to two antigenically distinct ribonucleoproteins of 60 kDa and 52 kDa. Three laboratory tests may be used to detect anti-Ro/SSA antibodies: counterimmunoelectrophoresis (CIE), enzyme- linked immunosorbent assay (ELISA) and immunoblotting (IB). Their relative efficacy and clinical correlations were ascertained. We determined anti- Ro/SSA antibodies with CIE, with two different ELISA methods (ELISA 1 and 2) and with lB in 29 SCLE patients. Anti-52 kDa and -60 kDa Ro/SSA antibodies were also assayed with IB. In addition, we determined antinuclear antibodies with indirect immunofluorescence, anti-Sm, anti-RNP, anti-La/SSB and anti- Ro/SSA antibodies with CIE and ELISA, and anti-nDNA and cardiolipin antibodies using an ELISA method. CIE detected anti-Ro/SSA antibodies in 22 patients while ELISA 1 and 2 did so in 17 and 18 patients, respectively. In live patients, IB revealed a reactivity to 60kDa polypeptides and in two, a reactivity to 52kDa polypeptides. Of these seven patients, four had a myocardial infarction. Of these, two reacted to the 52 kDa antigen and two to the 60 kDa antigen. A combination of techniques was often needed to detect all specificities. ELISA proved to be very specific and sensitive. The IB technique detected a group of patients with myocardial infarction. A case- control study is needed to confirm the data of cardiac involvement.

Original languageEnglish
Pages (from-to)114-117
Number of pages4
JournalBritish Journal of Dermatology
Volume138
Issue number1
DOIs
Publication statusPublished - 1998

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Cutaneous Lupus Erythematosus
Counterimmunoelectrophoresis
Immunoblotting
Enzyme-Linked Immunosorbent Assay
Myocardial Infarction
Antigens
Peptides
Cardiolipins
Ribonucleoproteins
Antinuclear Antibodies
Indirect Fluorescent Antibody Technique
SS-A antibodies
Case-Control Studies
Antibodies

ASJC Scopus subject areas

  • Dermatology

Cite this

Counterimmunoelectrophoresis, ELISA and immunoblotting detection of anti-Ro/SSA antibodies in subacute cutaneous lupus erythematosus. A comparative study. / Parodi, A.; Drosera, M.; Barbieri, L.; Rebora, A.

In: British Journal of Dermatology, Vol. 138, No. 1, 1998, p. 114-117.

Research output: Contribution to journalArticle

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