Counting the Nucleolar Organizer Region—Associated Proteins Is a Prognostic Clue of Malignant Melanoma

Claudio Gambini, Stefania Casazza, Luigi Borgiani, Marco Canepa, Salvatore Rovida, Franco Rongioletti, Alfredo Rebora

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Background and Design.— The nucleolar organizer regions (NORs) are chromosomal loops of DNA to which acidic proteins are associated that are seen by silver staining as black dots within the nucleoli (hereafter, these silver-staining NORs will be referred to as AgNORs). As their size and number reflect cell and nuclear activity, their counting in paraffin sections is regarded as a useful tool for diagnosing and prognosing malignant tumors. We counted AgNORs in 98 patients with stage I melanoma, followed up to an average of 73 months, to verify whether the number of AgNORs is of prognostic value. Results.— The number of AgNORs averaged 2.792 ±0.901 in the 64 patients without metastases and 4.889 ± 1.403 in the 34 with metastases. In patients with counts higher than 3.62, there was an 82% probability of metastases developing. Conclusion.— The technique is fast, simple, and reproducible with easily available reagents and standard light microscopy. In our population, AgNOR counts constituted a more accurate prognostic indicator than Clark's level and Breslow's thickness. Confirmation in a new population is needed.

Original languageEnglish
Pages (from-to)487-490
Number of pages4
JournalArchives of Dermatology
Volume128
Issue number4
DOIs
Publication statusPublished - 1992

Fingerprint

Nucleolus Organizer Region
Melanoma
Silver Staining
Neoplasm Metastasis
Proteins
Paraffin
Population
Microscopy
Cell Count
nucleolar organizer region associated proteins
Light
DNA
Neoplasms

ASJC Scopus subject areas

  • Dermatology

Cite this

Gambini, C., Casazza, S., Borgiani, L., Canepa, M., Rovida, S., Rongioletti, F., & Rebora, A. (1992). Counting the Nucleolar Organizer Region—Associated Proteins Is a Prognostic Clue of Malignant Melanoma. Archives of Dermatology, 128(4), 487-490. https://doi.org/10.1001/archderm.1992.01680140067004

Counting the Nucleolar Organizer Region—Associated Proteins Is a Prognostic Clue of Malignant Melanoma. / Gambini, Claudio; Casazza, Stefania; Borgiani, Luigi; Canepa, Marco; Rovida, Salvatore; Rongioletti, Franco; Rebora, Alfredo.

In: Archives of Dermatology, Vol. 128, No. 4, 1992, p. 487-490.

Research output: Contribution to journalArticle

Gambini, C, Casazza, S, Borgiani, L, Canepa, M, Rovida, S, Rongioletti, F & Rebora, A 1992, 'Counting the Nucleolar Organizer Region—Associated Proteins Is a Prognostic Clue of Malignant Melanoma', Archives of Dermatology, vol. 128, no. 4, pp. 487-490. https://doi.org/10.1001/archderm.1992.01680140067004
Gambini, Claudio ; Casazza, Stefania ; Borgiani, Luigi ; Canepa, Marco ; Rovida, Salvatore ; Rongioletti, Franco ; Rebora, Alfredo. / Counting the Nucleolar Organizer Region—Associated Proteins Is a Prognostic Clue of Malignant Melanoma. In: Archives of Dermatology. 1992 ; Vol. 128, No. 4. pp. 487-490.
@article{cb9f0f91a7b54cbe9d393f0c3432a30a,
title = "Counting the Nucleolar Organizer Region—Associated Proteins Is a Prognostic Clue of Malignant Melanoma",
abstract = "Background and Design.— The nucleolar organizer regions (NORs) are chromosomal loops of DNA to which acidic proteins are associated that are seen by silver staining as black dots within the nucleoli (hereafter, these silver-staining NORs will be referred to as AgNORs). As their size and number reflect cell and nuclear activity, their counting in paraffin sections is regarded as a useful tool for diagnosing and prognosing malignant tumors. We counted AgNORs in 98 patients with stage I melanoma, followed up to an average of 73 months, to verify whether the number of AgNORs is of prognostic value. Results.— The number of AgNORs averaged 2.792 ±0.901 in the 64 patients without metastases and 4.889 ± 1.403 in the 34 with metastases. In patients with counts higher than 3.62, there was an 82{\%} probability of metastases developing. Conclusion.— The technique is fast, simple, and reproducible with easily available reagents and standard light microscopy. In our population, AgNOR counts constituted a more accurate prognostic indicator than Clark's level and Breslow's thickness. Confirmation in a new population is needed.",
author = "Claudio Gambini and Stefania Casazza and Luigi Borgiani and Marco Canepa and Salvatore Rovida and Franco Rongioletti and Alfredo Rebora",
year = "1992",
doi = "10.1001/archderm.1992.01680140067004",
language = "English",
volume = "128",
pages = "487--490",
journal = "Archives of Dermatology",
issn = "0003-987X",
publisher = "American Medical Association",
number = "4",

}

TY - JOUR

T1 - Counting the Nucleolar Organizer Region—Associated Proteins Is a Prognostic Clue of Malignant Melanoma

AU - Gambini, Claudio

AU - Casazza, Stefania

AU - Borgiani, Luigi

AU - Canepa, Marco

AU - Rovida, Salvatore

AU - Rongioletti, Franco

AU - Rebora, Alfredo

PY - 1992

Y1 - 1992

N2 - Background and Design.— The nucleolar organizer regions (NORs) are chromosomal loops of DNA to which acidic proteins are associated that are seen by silver staining as black dots within the nucleoli (hereafter, these silver-staining NORs will be referred to as AgNORs). As their size and number reflect cell and nuclear activity, their counting in paraffin sections is regarded as a useful tool for diagnosing and prognosing malignant tumors. We counted AgNORs in 98 patients with stage I melanoma, followed up to an average of 73 months, to verify whether the number of AgNORs is of prognostic value. Results.— The number of AgNORs averaged 2.792 ±0.901 in the 64 patients without metastases and 4.889 ± 1.403 in the 34 with metastases. In patients with counts higher than 3.62, there was an 82% probability of metastases developing. Conclusion.— The technique is fast, simple, and reproducible with easily available reagents and standard light microscopy. In our population, AgNOR counts constituted a more accurate prognostic indicator than Clark's level and Breslow's thickness. Confirmation in a new population is needed.

AB - Background and Design.— The nucleolar organizer regions (NORs) are chromosomal loops of DNA to which acidic proteins are associated that are seen by silver staining as black dots within the nucleoli (hereafter, these silver-staining NORs will be referred to as AgNORs). As their size and number reflect cell and nuclear activity, their counting in paraffin sections is regarded as a useful tool for diagnosing and prognosing malignant tumors. We counted AgNORs in 98 patients with stage I melanoma, followed up to an average of 73 months, to verify whether the number of AgNORs is of prognostic value. Results.— The number of AgNORs averaged 2.792 ±0.901 in the 64 patients without metastases and 4.889 ± 1.403 in the 34 with metastases. In patients with counts higher than 3.62, there was an 82% probability of metastases developing. Conclusion.— The technique is fast, simple, and reproducible with easily available reagents and standard light microscopy. In our population, AgNOR counts constituted a more accurate prognostic indicator than Clark's level and Breslow's thickness. Confirmation in a new population is needed.

UR - http://www.scopus.com/inward/record.url?scp=0026519369&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026519369&partnerID=8YFLogxK

U2 - 10.1001/archderm.1992.01680140067004

DO - 10.1001/archderm.1992.01680140067004

M3 - Article

C2 - 1580655

AN - SCOPUS:0026519369

VL - 128

SP - 487

EP - 490

JO - Archives of Dermatology

JF - Archives of Dermatology

SN - 0003-987X

IS - 4

ER -