Coupling hydroxyapatite nanocrystals with lactoferrin as a promising strategy to fine regulate bone homeostasis

Monica Montesi, Silvia Panseri, Michele Iafisco, Alessio Adamiano, Anna Tampieri

Research output: Contribution to journalArticle

Abstract

Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis. In a previous study we observed that the coupling of LF and biomimetic hydroxyapatite nanocrystals (HA), a material well-known for its bioactivity and osteoconductive properties, leads to a combined effect in the induction of osteogenic differentiation of mesenchymal stem cells. On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis. Biomimetic HA nanocrystals were synthesized and functionalized with LF (HA-LF) and then pre-osteoblasts (MC3T3-E1) and monocyte/macrophage cells lines (RAW 264.7), using as osteoclastogenesis in vitro model, were cultured separately or in co-culture in presence of HA-LF. The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

Original languageEnglish
Article numbere0132633
JournalPLoS One
Volume10
Issue number7
DOIs
Publication statusPublished - Jul 6 2015

Fingerprint

nanocrystals
hydroxyapatite
Lactoferrin
lactoferrin
Durapatite
Nanoparticles
Nanocrystals
homeostasis
Bone
Homeostasis
bones
Bone and Bones
biomimetics
Biomimetics
osteoblasts
Osteoblasts
Bone Matrix
osteoclasts
Bone Remodeling
Macrophages

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Coupling hydroxyapatite nanocrystals with lactoferrin as a promising strategy to fine regulate bone homeostasis. / Montesi, Monica; Panseri, Silvia; Iafisco, Michele; Adamiano, Alessio; Tampieri, Anna.

In: PLoS One, Vol. 10, No. 7, e0132633, 06.07.2015.

Research output: Contribution to journalArticle

Montesi, Monica ; Panseri, Silvia ; Iafisco, Michele ; Adamiano, Alessio ; Tampieri, Anna. / Coupling hydroxyapatite nanocrystals with lactoferrin as a promising strategy to fine regulate bone homeostasis. In: PLoS One. 2015 ; Vol. 10, No. 7.
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