Cpx two-component signal transduction in Escherichia coli: Excessive CpxR-P levels underlie CpxA* phenotypes

Peter De Wulf, E. C C Lin

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

In Escherichia coli, the CpxA-CpxR two-component signal transduction system and the σ(E) and σ32 response pathways jointly regulate gene expression in adaptation to adverse conditions. These include envelope protein distress, heat shock, oxidative stress, high pH, and entry into stationary phase. Certain mutant versions of the CpxA sensor protein (CpxA* proteins) exhibit an elevated ratio of kinase to phosphatase activity on CpxR, the cognate response regulator. As a result, CpxA* strains display numerous phenotypes, many of which cannot be easily related to currently known functions of the CpxA-CpxR pathway. It is unclear whether CpxA* phenotypes are caused solely by hyperphosphorylation of CpxR. We here report that all of the tested CpxA* phenotypes depend on elevated levels of CpxR-P and not on cross-signalling of CpxA* to noncognate response regulators.

Original languageEnglish
Pages (from-to)1423-1426
Number of pages4
JournalJournal of Bacteriology
Volume182
Issue number5
DOIs
Publication statusPublished - Mar 2000

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Signal Transduction
Escherichia coli
Phenotype
Heat-Shock Proteins
Phosphoric Monoester Hydrolases
Oxidative Stress
Phosphotransferases
Gene Expression
Proteins

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Cpx two-component signal transduction in Escherichia coli : Excessive CpxR-P levels underlie CpxA* phenotypes. / De Wulf, Peter; Lin, E. C C.

In: Journal of Bacteriology, Vol. 182, No. 5, 03.2000, p. 1423-1426.

Research output: Contribution to journalArticle

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