Creatinine determination in serum by capillary electrophoresis

Rita Paroni, Isabella Fermo, Giuliana Cighetti, Carlo Alberto Ferrero, Anna Carobene, Ferruccio Ceriotti

Research output: Contribution to journalArticle

Abstract

Creatinine in human serum was separated in a fused-silica capillary with H3PO4 (75 mmol/L, pH 2.5) as BGE, followed by UV detection at 200 nm. Serum with methylimidazole added as internal standard was deproteinized with acetonitrile and the supernatant, after dilution with water was injected at pressure mode. Creatinine and methylimidazole were baseline-resolved in 6.5 min. Linearity in the 0-880 μmol/L range gave an r2 ≥ 0.998, recovery was 102 ± 2.8% (n = 6). Enzymatic breakdown with creatininase confirmed that serum does not interfere. The within-day and between-days coefficient of variation (CV) were

Original languageEnglish
Pages (from-to)463-468
Number of pages6
JournalElectrophoresis
Volume25
Issue number3
DOIs
Publication statusPublished - Feb 2004

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Keywords

  • Capillary electrophoresis
  • Creatinine
  • High-performance liquid chromatography
  • Serum

ASJC Scopus subject areas

  • Clinical Biochemistry

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