CREB selectively controls learning-induced structural remodeling of neurons

Silvia Middei, Alida Spalloni, Patrizia Longone, Christopher Pittenger, Shane M. O'Mara, Hélène Marie, Martine Ammassari-Teule

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The modulation of synaptic strength associated with learning is post-synaptically regulated by changes in density and shape of dendritic spines. The transcription factor CREB (cAMP response element binding protein) is required for memory formation and in vitro dendritic spine rearrangements, but its role in learning-induced remodeling of neurons remains elusive. Using transgenic mice conditionally expressing a dominant-negative CREB (CREBS133A: mCREB) mutant, we found that inhibiting CREB function does not alter spine density, spine morphology, and levels of polymerized actin in naive CA1 neurons. CREB inhibition, however, impaired contextual fear conditioning and produced a learning-induced collapse of spines associated with a blockade of learning-dependent increase in actin polymerization. Blocking mCREB expression with doxycycline rescued memory and restored a normal pattern of learning-induced spines, demonstrating that CREB controls structural adaptations of neurons selectively involved in memory formation.

Original languageEnglish
Pages (from-to)330-336
Number of pages7
JournalLearning and Memory
Volume19
Issue number8
DOIs
Publication statusPublished - Aug 2012

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Cyclic AMP Response Element-Binding Protein
Learning
Neurons
Spine
Dendritic Spines
Actins
Doxycycline
Polymerization
Transgenic Mice
Fear
Transcription Factors

ASJC Scopus subject areas

  • Cognitive Neuroscience
  • Cellular and Molecular Neuroscience
  • Neuropsychology and Physiological Psychology

Cite this

CREB selectively controls learning-induced structural remodeling of neurons. / Middei, Silvia; Spalloni, Alida; Longone, Patrizia; Pittenger, Christopher; O'Mara, Shane M.; Marie, Hélène; Ammassari-Teule, Martine.

In: Learning and Memory, Vol. 19, No. 8, 08.2012, p. 330-336.

Research output: Contribution to journalArticle

Middei, S, Spalloni, A, Longone, P, Pittenger, C, O'Mara, SM, Marie, H & Ammassari-Teule, M 2012, 'CREB selectively controls learning-induced structural remodeling of neurons', Learning and Memory, vol. 19, no. 8, pp. 330-336. https://doi.org/10.1101/lm.025817.112
Middei, Silvia ; Spalloni, Alida ; Longone, Patrizia ; Pittenger, Christopher ; O'Mara, Shane M. ; Marie, Hélène ; Ammassari-Teule, Martine. / CREB selectively controls learning-induced structural remodeling of neurons. In: Learning and Memory. 2012 ; Vol. 19, No. 8. pp. 330-336.
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