Abstract
The modulation of synaptic strength associated with learning is post-synaptically regulated by changes in density and shape of dendritic spines. The transcription factor CREB (cAMP response element binding protein) is required for memory formation and in vitro dendritic spine rearrangements, but its role in learning-induced remodeling of neurons remains elusive. Using transgenic mice conditionally expressing a dominant-negative CREB (CREBS133A: mCREB) mutant, we found that inhibiting CREB function does not alter spine density, spine morphology, and levels of polymerized actin in naive CA1 neurons. CREB inhibition, however, impaired contextual fear conditioning and produced a learning-induced collapse of spines associated with a blockade of learning-dependent increase in actin polymerization. Blocking mCREB expression with doxycycline rescued memory and restored a normal pattern of learning-induced spines, demonstrating that CREB controls structural adaptations of neurons selectively involved in memory formation.
| Original language | English |
|---|---|
| Pages (from-to) | 330-336 |
| Number of pages | 7 |
| Journal | Learning and Memory |
| Volume | 19 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Aug 2012 |
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ASJC Scopus subject areas
- Cognitive Neuroscience
- Cellular and Molecular Neuroscience
- Neuropsychology and Physiological Psychology
Cite this
CREB selectively controls learning-induced structural remodeling of neurons. / Middei, Silvia; Spalloni, Alida; Longone, Patrizia; Pittenger, Christopher; O'Mara, Shane M.; Marie, Hélène; Ammassari-Teule, Martine.
In: Learning and Memory, Vol. 19, No. 8, 08.2012, p. 330-336.Research output: Contribution to journal › Article
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TY - JOUR
T1 - CREB selectively controls learning-induced structural remodeling of neurons
AU - Middei, Silvia
AU - Spalloni, Alida
AU - Longone, Patrizia
AU - Pittenger, Christopher
AU - O'Mara, Shane M.
AU - Marie, Hélène
AU - Ammassari-Teule, Martine
PY - 2012/8
Y1 - 2012/8
N2 - The modulation of synaptic strength associated with learning is post-synaptically regulated by changes in density and shape of dendritic spines. The transcription factor CREB (cAMP response element binding protein) is required for memory formation and in vitro dendritic spine rearrangements, but its role in learning-induced remodeling of neurons remains elusive. Using transgenic mice conditionally expressing a dominant-negative CREB (CREBS133A: mCREB) mutant, we found that inhibiting CREB function does not alter spine density, spine morphology, and levels of polymerized actin in naive CA1 neurons. CREB inhibition, however, impaired contextual fear conditioning and produced a learning-induced collapse of spines associated with a blockade of learning-dependent increase in actin polymerization. Blocking mCREB expression with doxycycline rescued memory and restored a normal pattern of learning-induced spines, demonstrating that CREB controls structural adaptations of neurons selectively involved in memory formation.
AB - The modulation of synaptic strength associated with learning is post-synaptically regulated by changes in density and shape of dendritic spines. The transcription factor CREB (cAMP response element binding protein) is required for memory formation and in vitro dendritic spine rearrangements, but its role in learning-induced remodeling of neurons remains elusive. Using transgenic mice conditionally expressing a dominant-negative CREB (CREBS133A: mCREB) mutant, we found that inhibiting CREB function does not alter spine density, spine morphology, and levels of polymerized actin in naive CA1 neurons. CREB inhibition, however, impaired contextual fear conditioning and produced a learning-induced collapse of spines associated with a blockade of learning-dependent increase in actin polymerization. Blocking mCREB expression with doxycycline rescued memory and restored a normal pattern of learning-induced spines, demonstrating that CREB controls structural adaptations of neurons selectively involved in memory formation.
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U2 - 10.1101/lm.025817.112
DO - 10.1101/lm.025817.112
M3 - Article
C2 - 22815537
AN - SCOPUS:84865202866
VL - 19
SP - 330
EP - 336
JO - Learning and Memory
JF - Learning and Memory
SN - 1072-0502
IS - 8
ER -