CREM-dependent transcription in male germ cells controlled by a kinesin

Betina Macho, Stefano Brancorsini, Gian Maria Fimia, Mitsutoshi Setou, Nobutaka Hirokawa, Paolo Sassone-Corsi

Research output: Contribution to journalArticle

Abstract

ACT is a LIM-only protein expressed exclusively in round spermatids, where it cooperates with transcriptional activator CREM in regulating various postmeiotic genes. Targeted inactivation of CREM leads to a complete block of mouse spermiogenesis. We sought to identify the regulatory steps controlling the functional interplay between CREM and ACT. We found that ACT selectively associates with KIF17b, a kinesin highly expressed in male germ cells. The ACT-KIF17b interaction is restricted to specific stages of spermatogenesis and directly determines the intracellular localization of ACT. Sensitivity to leptomycin B indicates that KIF17b can be actively exported from the nucleus through the Crml receptor. Thus, a kinesin directly controls the activity of a transcriptional coactivator by a tight regulation of its intracellular localization.

Original languageEnglish
Pages (from-to)2388-2390
Number of pages3
JournalScience
Volume298
Issue number5602
DOIs
Publication statusPublished - Dec 20 2002

ASJC Scopus subject areas

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    Macho, B., Brancorsini, S., Fimia, G. M., Setou, M., Hirokawa, N., & Sassone-Corsi, P. (2002). CREM-dependent transcription in male germ cells controlled by a kinesin. Science, 298(5602), 2388-2390. https://doi.org/10.1126/science.1077265