Creutzfeldt-Jakob disease: Carnoy's fixative improves the immunohistochemistry of the proteinase K-resistant prion protein

Giorgio Giaccone, Barbara Canciani, Gianfranco Puoti, Giacomina Rossi, Donato Goffredo, Selina Iussich, Paolo Fociani, Fabrizio Tagliavini, Orso Bugiani

Research output: Contribution to journalArticle

Abstract

The neuropathological diagnosis of Creutzfeldt-Jakob disease relies on the immunohistochemical demonstration of the proteinase-K resistant form of the prion protein (PrP(res)) in the brain tissue. The antigenicity of PrP(res) is strongly reduced by the formalin solution widely used to fix the tissue, thus the PrP(res) immunoreactivity is inconsistently detectable in formalin-fixed tissue. A better PrP(res) immunostaining can be obtained by using Carnoy's fixing solution, which is composed of ethanol, chloroform and acetic acid (6:3:1). PrP(res) can easily be extracted from Carnoy's-fixed, paraplast-embedded tissue. Accordingly, Carnoy's-fixed tissue can prior to immunolabeling be subjected to proteinase K and guanidine thiocyanate, which respectively eliminate the normal cellular form of prion protein and promote protein denaturation. In comparison with the best protocols for formalin- fixed tissue (i.e. - hydrolytic autoclaving or autoclaving in distilled water followed by formic acid and guanidine thiocyanate), PrP(res) immunostaining carried out on sections cut from Carnoy's-fixed, paraplast-embedded tissue blocks and subjected to proteinase K and guanidine thiocyanate, proved more successful to detect and map both diffuse and focal PrP(res) immunoreactivity, and to correlate the immunoreactivity pattern with MV polymorphism at PRNP codon 129 and PrP(res) banding and glycosylation pattern revealed by Western blot.

Original languageEnglish
Pages (from-to)31-37
Number of pages7
JournalBrain Pathology
Volume10
Issue number1
Publication statusPublished - Jan 2000

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PrPSc Proteins
Endopeptidase K
Fixatives
Creutzfeldt-Jakob Syndrome
Immunohistochemistry
Formaldehyde
formic acid
Protein Denaturation
Prion Proteins
Chloroform
Glycosylation
Codon
Acetic Acid
Ethanol
Western Blotting

ASJC Scopus subject areas

  • Neuroscience(all)
  • Pathology and Forensic Medicine

Cite this

Giaccone, G., Canciani, B., Puoti, G., Rossi, G., Goffredo, D., Iussich, S., ... Bugiani, O. (2000). Creutzfeldt-Jakob disease: Carnoy's fixative improves the immunohistochemistry of the proteinase K-resistant prion protein. Brain Pathology, 10(1), 31-37.

Creutzfeldt-Jakob disease : Carnoy's fixative improves the immunohistochemistry of the proteinase K-resistant prion protein. / Giaccone, Giorgio; Canciani, Barbara; Puoti, Gianfranco; Rossi, Giacomina; Goffredo, Donato; Iussich, Selina; Fociani, Paolo; Tagliavini, Fabrizio; Bugiani, Orso.

In: Brain Pathology, Vol. 10, No. 1, 01.2000, p. 31-37.

Research output: Contribution to journalArticle

Giaccone, G, Canciani, B, Puoti, G, Rossi, G, Goffredo, D, Iussich, S, Fociani, P, Tagliavini, F & Bugiani, O 2000, 'Creutzfeldt-Jakob disease: Carnoy's fixative improves the immunohistochemistry of the proteinase K-resistant prion protein', Brain Pathology, vol. 10, no. 1, pp. 31-37.
Giaccone, Giorgio ; Canciani, Barbara ; Puoti, Gianfranco ; Rossi, Giacomina ; Goffredo, Donato ; Iussich, Selina ; Fociani, Paolo ; Tagliavini, Fabrizio ; Bugiani, Orso. / Creutzfeldt-Jakob disease : Carnoy's fixative improves the immunohistochemistry of the proteinase K-resistant prion protein. In: Brain Pathology. 2000 ; Vol. 10, No. 1. pp. 31-37.
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