Critical defect healing assessment in rat calvaria filled with injectable calcium phosphate cement

Luis Eduardo Schmidt, Henrique Hadad, Igor Rodrigues De Vasconcelos, Luara Teixeira Colombo, Rodrigo Capalbo Da Silva, Ana Flavia Piquera Santos, Lara Cristina Cunha Cervantes, Pier Paolo Poli, Fabrizio Signorino, Carlo Maiorana, Paulo Sérgio Perri De Carvalho, Francisley Ávila Souza

Research output: Contribution to journalArticlepeer-review


(1) Background: The tissue engineering field has been working to find biomaterials that mimic the biological properties of autogenous bone grafts. (2) Aim: To evaluate the osteoconduction potential of injectable calcium phosphate cement implanted in critical defects in rat calvaria. (3) Methods: In the calvarial bone of 36 rats, 7-mm diameter critical size defects were performed. Afterwards, the animals were randomly divided into three groups according to filler material: a blood clot group (BC), blood clot membrane group (BCM), and an injectable β-tricalcium phosphate group (HBS) cement group. After periods of 30 and 60 days, the animals were euthanized, the calvaria was isolated, and submitted to a decalcification process for later blades confection. Qualitative and quantitative analysis of the neoformed bone tissue were conducted, and histometric data were statistically analyzed. (4) Results: Sixty days post-surgery, the percentages of neoformed bone were 10.67 ± 5.57 in group BC, 16.71 ± 5.0 in group BCM, and 55.11 ± 13.20 in group HBS. The bone formation values in group HBS were significantly higher (p < 0.05) than in groups BC and BCM. (5) Conclusions: Based on these results, it can be concluded that injectable calcium phosphate cement is an osteoconductive material that can be used to fill bone cavities.

Original languageEnglish
Article number21
JournalJournal of Functional Biomaterials
Issue number2
Publication statusPublished - Jan 1 2019


  • Biomaterial
  • Bone healing
  • Calcium phosphate cement

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering


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