Dendritic cells (DCs) need activation for the priming of antigen-specific immune responses. Recently activated Des were described to prime in vitro strong T helper cell type 1 (Th1) responses, whereas at later time points, the same cells preferentially prime Th2 cells [Langenkemp, A. et al., Nat. Immunol. 1: 311-316, 2000]. Because the immune response against cancer strongly depends on CTLs of Th1-like phenotype (Tc1), we verified here whether the kinetics of DCs activation also impacted on in vivo priming of tumor-specific CTLs. After pulsing with the CTL epitope TRP-2180-188, bone-marrow-derived DCs, exposed to lipopolysaccharide (LPS) for 8 h (8hDC), elicited a more powerful Tc1 response in C57BL/6 mice than did untreated DCs, or DCs exposed to LPS for 48 h (48hDC). Indeed, 8hDCs were the most potent protective and therapeutic vaccine against B16 melanoma. Despite a higher expression of MHC and costimulatory molecules by 48hDCs, 8hDCs and 48hDCs showed comparable allostimulatory and migration potential, and susceptibility to CTL-mediated apoptosis. However, 8hDCs exhibited a significantly higher interleukin (IL)-12 production potential. Release of IL-12 was necessary to induce potent Tc1 cells, because DCs from IL-12p40-/- mice, irrespective of their maturation level, generated low CTL responses, comparable with 48hDCs and OhDCs from wild-type animals. Our data are relevant for the design of DC-based vaccines.
|Number of pages||7|
|Publication status||Published - Jul 1 2003|
ASJC Scopus subject areas
- Cancer Research