Critical role of multidimensional flow cytometry in detecting occult leptomeningeal disease in newly diagnosed aggressive B-cell lymphomas

R. Di Noto, G. Scalia, G. Abate, M. Gorrese, C. Pascariello, M. Raia, P. Morabito, F. Capone, C. Lo Pardo, P. Mirabelli, E. Mariotti, L. Del Vecchio

Research output: Contribution to journalArticle

Abstract

Among histological aggressive non-Hodgkin lymphomas (NHL), the overall risk of central nervous system (CNS) relapse is approximately 5%, a figure which is too low to offer prophylaxis to all patients. The aim of this work is to demonstrate the utility of flow cytometry (FCM) in detecting occult leptomeningeal disease in this subtype of NHL. We studied cerebrospinal fluid (CSF) involvement in 42 newly diagnosed aggressive NHL patients at risk for CNS involvement. We used multicolour FCM to detect CSF infiltrating neoplastic cells. Among the 42 patients studied, 11 had CSF involvement as detected by FCM. Of these, only four were also positive for conventional morphology (p = 0.046). These results designate that FCM as the first choice technique in NHL CSF clinical cell analysis.

Original languageEnglish
Pages (from-to)1196-1199
Number of pages4
JournalLeukemia Research
Volume32
Issue number8
DOIs
Publication statusPublished - Aug 2008

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Keywords

  • Cerebrospinal fluid
  • Flow cytometry
  • Non-Hodgkin lymphoma

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Di Noto, R., Scalia, G., Abate, G., Gorrese, M., Pascariello, C., Raia, M., Morabito, P., Capone, F., Pardo, C. L., Mirabelli, P., Mariotti, E., & Del Vecchio, L. (2008). Critical role of multidimensional flow cytometry in detecting occult leptomeningeal disease in newly diagnosed aggressive B-cell lymphomas. Leukemia Research, 32(8), 1196-1199. https://doi.org/10.1016/j.leukres.2007.12.016