Cross-clade HIV-1-specific neutralizing IgA in mucosal and systemic compartments of HIV-1-exposed, persistently seronegative subjects

Claudia Devito, Jorma Hinkula, Rupert Kaul, Joshua Kimani, Peter Kiama, Lucia Lopalco, Claudia Barass, Stefania Piconi, Daria Trabattoni, Job J. Bwayo, Francis Plummer, Mario Clerici, Kristina Broliden

Research output: Contribution to journalArticle

Abstract

There is an urgent need for a universally effective HIV-1 vaccine, but whether a vaccine will be able to protect against HIV-1 of different clades is a significant concern. IgA from HIV-1-exposed, persistently seronegative (HEPS) subjects has been shown to neutralize HIV-1 and to block epithelial HIV-1 transcytosis, and it may target novel HIV-1 epitopes. We have tested the ability of plasma and mucosal IgA purified from HEPS subjects to neutralize HIV-1 primary isolates of different viral clades and phenotypes. IgA from two groups of HEPS subjects was tested: sex workers from Nairobi, Kenya, where clades A and D predominate, and the heterosexual partners of individuals infected by clade B virus. HIV-1-infected and low-risk uninfected individuals were included as controls. IgA purified from the blood, genital tract, and saliva of most HEPS sex workers demonstrated significant crossclade HIV-1 neutralization, whereas a more clade-restricted pattern of neutralization was found in partners of clade B-infected individuals. IgA purified from HIV-1-infected individuals also mediated cross-clade neutralization, whereas IgA from uninfected controls lacked neutralizing activity. In conclusion, mucosal and plasma IgA from HEPS subjects neutralizes HIV-1 of different clades. This ability to induce HIV-1-specific systemic and mucosal IgA may be an important feature of an effective prophylactic HIV-1 vaccine.

Original languageEnglish
Pages (from-to)413-420
Number of pages8
JournalJournal of Acquired Immune Deficiency Syndromes
Volume30
Issue number4
Publication statusPublished - Aug 1 2002

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Keywords

  • Exposed
  • HIV-1 cross-clade
  • IgA
  • Neutralization
  • Uninfected

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Devito, C., Hinkula, J., Kaul, R., Kimani, J., Kiama, P., Lopalco, L., Barass, C., Piconi, S., Trabattoni, D., Bwayo, J. J., Plummer, F., Clerici, M., & Broliden, K. (2002). Cross-clade HIV-1-specific neutralizing IgA in mucosal and systemic compartments of HIV-1-exposed, persistently seronegative subjects. Journal of Acquired Immune Deficiency Syndromes, 30(4), 413-420.