Cross evaluation of three flow cytometric F cell counting methods performed by different laboratories

G. S. Paterakis, S. L. Thein, E. Fibach, M. D. Cappellini

Research output: Contribution to journalArticle

Abstract

Three flow cytometric methods of counting F cells were evaluated in the settings of an external laboratory assessment scheme. The laboratories to participate with a different method were located in Oxford (method O), Athens (method A) and Jerusalem, (method J). Two monoclonal anti-γ chain antibodies were used: monoclonal antibody produced by P. Beverley (Oxford) (BEV) and an antibody provided by Bioatlantic S.A.R.L. (France) (BIO). The specimens tested were mixtures in five predefined ratios of a sample with homozygous δβ-thalassemia with 100% F cells with a sample with no F cells. A central independent laboratory prepared and distributed the aliquots (at room temperature) to the participating centers within 2 (O), 3 (A), and 6 (J) days. The performance of the three methods was evaluated by: 1) deviation indices, 2) relative accuracy, as percent difference of the counts from the target values, and 3) bias and linearity by linear regression of the counts versus the target values [parameters: slope (s), y-intercept (y), R squared (Rs), and F ratio]. The highest score of performance was obtained by method A with both monoclonal antibodies.

Original languageEnglish
Pages (from-to)427-444
Number of pages18
JournalHemoglobin
Volume22
Issue number5-6
Publication statusPublished - 1998

Keywords

  • Erythrocytes
  • F cells
  • F erythrocytes
  • Fetal cells
  • Flow cytometry
  • Hb F
  • Methods
  • RBC
  • Red cells
  • Techniques

ASJC Scopus subject areas

  • Hematology
  • Biochemistry

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