Cross-linking of at least three binding sites mediates signal transduction in a CR2-positive Burkitt lymphoma derived cell line (Raji)

Guiseppe Barile, Barbara Pernozzoli, Valentina Tiracchia, Salvatore Ioppolo, Maria De Cristofaro, Elisabetta Mattei, Alberto Faggioni

Research output: Contribution to journalArticlepeer-review

Abstract

In this study we demonstrate that Raji cells, a CR2-positive Burkitt lymphoma-derived cell line, during cell growth, need the cross-linking of multiple OKB7 binding sites or C3d determinants to mediate signal transduction. The loss of one of these affects the cellular response. Moreover, OKB7, the anti-CR2 MoAb, recognizes C3d determinants on the cell surface and inhibits signal transduction induced by anti-C3d polyclonal antibody. Since Raji cells are always CR2 positive during cell growth, we suppose that at least another protein, along with CR2, may be involved in setting up a cell surface complex able to receive and transduce the signal triggered by OKB7. In our experimental system the protein that offers a third binding site to OKB7, may be represented by a 33 kDa protein bearing C3d determinants.

Original languageEnglish
Pages (from-to)291-296
Number of pages6
JournalFEBS Letters
Volume372
Issue number2-3
DOIs
Publication statusPublished - Sep 25 1995

Keywords

  • Anti-C3d reactive binding site
  • Anti-CR2 MoAb
  • Complement receptor type 2
  • Inositol-(1,4,5)trisphosphate
  • Polyclonal antibodies for C3c and C3d fragments of human complement (C3)

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

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