Abstract
In this study we demonstrate that Raji cells, a CR2-positive Burkitt lymphoma-derived cell line, during cell growth, need the cross-linking of multiple OKB7 binding sites or C3d determinants to mediate signal transduction. The loss of one of these affects the cellular response. Moreover, OKB7, the anti-CR2 MoAb, recognizes C3d determinants on the cell surface and inhibits signal transduction induced by anti-C3d polyclonal antibody. Since Raji cells are always CR2 positive during cell growth, we suppose that at least another protein, along with CR2, may be involved in setting up a cell surface complex able to receive and transduce the signal triggered by OKB7. In our experimental system the protein that offers a third binding site to OKB7, may be represented by a 33 kDa protein bearing C3d determinants.
Original language | English |
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Pages (from-to) | 291-296 |
Number of pages | 6 |
Journal | FEBS Letters |
Volume | 372 |
Issue number | 2-3 |
DOIs | |
Publication status | Published - Sep 25 1995 |
Keywords
- Anti-C3d reactive binding site
- Anti-CR2 MoAb
- Complement receptor type 2
- Inositol-(1,4,5)trisphosphate
- Polyclonal antibodies for C3c and C3d fragments of human complement (C3)
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Genetics
- Molecular Biology
- Structural Biology