Cross talk among Notch3, pre-TCR, and Tal1 in T-cell development and leukemogenesis

Claudio Talora, Samantha Cialfi, Christian Oliviero, Rocco Palermo, Monica Pascucci, Luigi Frati, Alessandra Vacca, Alberto Gulino, Isabella Screpanti

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Integrated pathways are believed to determine hematopoietic cell fate and/or neoplastic transformation. Notch signaling has been shown to regulate T-cell differentiation and leukemogenesis. However, specific target genes and molecular partners are not fully elucidated. We show that Notch3 activation sustains aberrant SCL/Tal1 overexpression and phosphorylation in mature thymocytes. Furthermore, we define the role of SCL/Tal1 as a component of an activator complex, including phosphorylated Tal1 and Sp1, that specifically enhances cyclin D1 expression and demonstrate that Tal1/Sp1 specifically co-occupy the D1 promoter in vivo, only in the presence of pre-T-cell receptor (TCR). We therefore conclude not only that cyclin D1 is a target of the Tal1/Sp1 complex, but also that Notch3-dependent activation of pre-TCR/ERK signaling regulates SCL/Tal1 function.

Original languageEnglish
Pages (from-to)3313-3320
Number of pages8
JournalBlood
Volume107
Issue number8
DOIs
Publication statusPublished - Apr 15 2006

Fingerprint

T-cells
Cyclin D1
T-Cell Antigen Receptor
Chemical activation
T-Lymphocytes
Phosphorylation
Thymocytes
Cell Differentiation
Genes

ASJC Scopus subject areas

  • Hematology

Cite this

Talora, C., Cialfi, S., Oliviero, C., Palermo, R., Pascucci, M., Frati, L., ... Screpanti, I. (2006). Cross talk among Notch3, pre-TCR, and Tal1 in T-cell development and leukemogenesis. Blood, 107(8), 3313-3320. https://doi.org/10.1182/blood-2005-07-2823

Cross talk among Notch3, pre-TCR, and Tal1 in T-cell development and leukemogenesis. / Talora, Claudio; Cialfi, Samantha; Oliviero, Christian; Palermo, Rocco; Pascucci, Monica; Frati, Luigi; Vacca, Alessandra; Gulino, Alberto; Screpanti, Isabella.

In: Blood, Vol. 107, No. 8, 15.04.2006, p. 3313-3320.

Research output: Contribution to journalArticle

Talora, C, Cialfi, S, Oliviero, C, Palermo, R, Pascucci, M, Frati, L, Vacca, A, Gulino, A & Screpanti, I 2006, 'Cross talk among Notch3, pre-TCR, and Tal1 in T-cell development and leukemogenesis', Blood, vol. 107, no. 8, pp. 3313-3320. https://doi.org/10.1182/blood-2005-07-2823
Talora C, Cialfi S, Oliviero C, Palermo R, Pascucci M, Frati L et al. Cross talk among Notch3, pre-TCR, and Tal1 in T-cell development and leukemogenesis. Blood. 2006 Apr 15;107(8):3313-3320. https://doi.org/10.1182/blood-2005-07-2823
Talora, Claudio ; Cialfi, Samantha ; Oliviero, Christian ; Palermo, Rocco ; Pascucci, Monica ; Frati, Luigi ; Vacca, Alessandra ; Gulino, Alberto ; Screpanti, Isabella. / Cross talk among Notch3, pre-TCR, and Tal1 in T-cell development and leukemogenesis. In: Blood. 2006 ; Vol. 107, No. 8. pp. 3313-3320.
@article{4261ff2c16cf4f50b7e1158f01eaed58,
title = "Cross talk among Notch3, pre-TCR, and Tal1 in T-cell development and leukemogenesis",
abstract = "Integrated pathways are believed to determine hematopoietic cell fate and/or neoplastic transformation. Notch signaling has been shown to regulate T-cell differentiation and leukemogenesis. However, specific target genes and molecular partners are not fully elucidated. We show that Notch3 activation sustains aberrant SCL/Tal1 overexpression and phosphorylation in mature thymocytes. Furthermore, we define the role of SCL/Tal1 as a component of an activator complex, including phosphorylated Tal1 and Sp1, that specifically enhances cyclin D1 expression and demonstrate that Tal1/Sp1 specifically co-occupy the D1 promoter in vivo, only in the presence of pre-T-cell receptor (TCR). We therefore conclude not only that cyclin D1 is a target of the Tal1/Sp1 complex, but also that Notch3-dependent activation of pre-TCR/ERK signaling regulates SCL/Tal1 function.",
author = "Claudio Talora and Samantha Cialfi and Christian Oliviero and Rocco Palermo and Monica Pascucci and Luigi Frati and Alessandra Vacca and Alberto Gulino and Isabella Screpanti",
year = "2006",
month = "4",
day = "15",
doi = "10.1182/blood-2005-07-2823",
language = "English",
volume = "107",
pages = "3313--3320",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "8",

}

TY - JOUR

T1 - Cross talk among Notch3, pre-TCR, and Tal1 in T-cell development and leukemogenesis

AU - Talora, Claudio

AU - Cialfi, Samantha

AU - Oliviero, Christian

AU - Palermo, Rocco

AU - Pascucci, Monica

AU - Frati, Luigi

AU - Vacca, Alessandra

AU - Gulino, Alberto

AU - Screpanti, Isabella

PY - 2006/4/15

Y1 - 2006/4/15

N2 - Integrated pathways are believed to determine hematopoietic cell fate and/or neoplastic transformation. Notch signaling has been shown to regulate T-cell differentiation and leukemogenesis. However, specific target genes and molecular partners are not fully elucidated. We show that Notch3 activation sustains aberrant SCL/Tal1 overexpression and phosphorylation in mature thymocytes. Furthermore, we define the role of SCL/Tal1 as a component of an activator complex, including phosphorylated Tal1 and Sp1, that specifically enhances cyclin D1 expression and demonstrate that Tal1/Sp1 specifically co-occupy the D1 promoter in vivo, only in the presence of pre-T-cell receptor (TCR). We therefore conclude not only that cyclin D1 is a target of the Tal1/Sp1 complex, but also that Notch3-dependent activation of pre-TCR/ERK signaling regulates SCL/Tal1 function.

AB - Integrated pathways are believed to determine hematopoietic cell fate and/or neoplastic transformation. Notch signaling has been shown to regulate T-cell differentiation and leukemogenesis. However, specific target genes and molecular partners are not fully elucidated. We show that Notch3 activation sustains aberrant SCL/Tal1 overexpression and phosphorylation in mature thymocytes. Furthermore, we define the role of SCL/Tal1 as a component of an activator complex, including phosphorylated Tal1 and Sp1, that specifically enhances cyclin D1 expression and demonstrate that Tal1/Sp1 specifically co-occupy the D1 promoter in vivo, only in the presence of pre-T-cell receptor (TCR). We therefore conclude not only that cyclin D1 is a target of the Tal1/Sp1 complex, but also that Notch3-dependent activation of pre-TCR/ERK signaling regulates SCL/Tal1 function.

UR - http://www.scopus.com/inward/record.url?scp=33645751939&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645751939&partnerID=8YFLogxK

U2 - 10.1182/blood-2005-07-2823

DO - 10.1182/blood-2005-07-2823

M3 - Article

VL - 107

SP - 3313

EP - 3320

JO - Blood

JF - Blood

SN - 0006-4971

IS - 8

ER -