Crosstalk between normal and tumoral brain cells: Effect on sex steroid metabolism

Roberto Cosimo Melcangi, Ilaria Cavarretta, Valerio Magnaghi, Marinella Ballabio, Luciano Martini, Marcella Motta

Research output: Contribution to journalArticle

Abstract

The present article shows for the first time that two cell lines derived respectively from a rat glioma (C6 cell line) and from a human astrocytoma (1321N1 cell line) are able to convert testosterone and progesterone into their corresponding 5α-reduced metabolites dihydrotestosterone and dihydroprogesterone. Moreover, both cell lines are also able to convert these metabolites further into their corresponding 3α-OH derivatives, 5α- androstan-3α, 7β-diol (3α-diol) and tetrahydroprogesterone. On the basis of these observations, the possibility that secretory products of normal and tumoral brain cells might be able to influence steroid metabolism occurring in the two glial cell lines previously mentioned as well as in fetal rat neurons and in neonatal rat type 1 astrocytes has been considered. To this purpose, cultures of the different cellular types have been exposed to the conditioned medium in which the other cells were grown. The results obtained indicate that: 1. Neurons are able to stimulate, in a statistically significant fashion, the formation of dihydrotestosterone (DHT), 3α-diol, and tetrahydraprogesterone (THP) in C6 cells. 2. Type 1 astrocytes, on the contrary, are unable to modify steroid metabolism in C6 cells. 3. C6 cell product(s) decrease(s) the formation of DHP in type 1 astrocytes, without modifying that of DHT. 4. C6 cells do not influence the metabolism of testosterone (T) and progesterone (P) in neurons. In conclusion, the present observations show that the conditioned medium of normal neurons is able to increase the metabolism of testosterone and progesterone occurring in a tumoral glial cell line, and that the conditioned media of the two tumoral cell lines analyzed are able to decrease the conversion of P into DHP occurring in normal type 1 astrocytes. The surprising result that these conditioned media do not alter the formation of DHT is discussed. Work is presently in progress to identify the principle(s) responsible respectively for the activating and inhibiting actions here described.

Original languageEnglish
Pages (from-to)65-71
Number of pages7
JournalEndocrine
Volume8
Issue number1
DOIs
Publication statusPublished - 1998

Fingerprint

Steroids
Dihydrotestosterone
Cell Line
Conditioned Culture Medium
Brain
Astrocytes
Progesterone
Testosterone
Neurons
Neuroglia
20-alpha-Dihydroprogesterone
Astrocytoma
Glioma

Keywords

  • Astrocytes
  • Human astrocytoma
  • Neurons
  • Progesterone
  • Rat glioma
  • Testosterone

ASJC Scopus subject areas

  • Endocrinology

Cite this

Melcangi, R. C., Cavarretta, I., Magnaghi, V., Ballabio, M., Martini, L., & Motta, M. (1998). Crosstalk between normal and tumoral brain cells: Effect on sex steroid metabolism. Endocrine, 8(1), 65-71. https://doi.org/10.1385/ENDO:8:1:65

Crosstalk between normal and tumoral brain cells : Effect on sex steroid metabolism. / Melcangi, Roberto Cosimo; Cavarretta, Ilaria; Magnaghi, Valerio; Ballabio, Marinella; Martini, Luciano; Motta, Marcella.

In: Endocrine, Vol. 8, No. 1, 1998, p. 65-71.

Research output: Contribution to journalArticle

Melcangi, RC, Cavarretta, I, Magnaghi, V, Ballabio, M, Martini, L & Motta, M 1998, 'Crosstalk between normal and tumoral brain cells: Effect on sex steroid metabolism', Endocrine, vol. 8, no. 1, pp. 65-71. https://doi.org/10.1385/ENDO:8:1:65
Melcangi RC, Cavarretta I, Magnaghi V, Ballabio M, Martini L, Motta M. Crosstalk between normal and tumoral brain cells: Effect on sex steroid metabolism. Endocrine. 1998;8(1):65-71. https://doi.org/10.1385/ENDO:8:1:65
Melcangi, Roberto Cosimo ; Cavarretta, Ilaria ; Magnaghi, Valerio ; Ballabio, Marinella ; Martini, Luciano ; Motta, Marcella. / Crosstalk between normal and tumoral brain cells : Effect on sex steroid metabolism. In: Endocrine. 1998 ; Vol. 8, No. 1. pp. 65-71.
@article{c04aa0dae49e4be8b186fc6856651644,
title = "Crosstalk between normal and tumoral brain cells: Effect on sex steroid metabolism",
abstract = "The present article shows for the first time that two cell lines derived respectively from a rat glioma (C6 cell line) and from a human astrocytoma (1321N1 cell line) are able to convert testosterone and progesterone into their corresponding 5α-reduced metabolites dihydrotestosterone and dihydroprogesterone. Moreover, both cell lines are also able to convert these metabolites further into their corresponding 3α-OH derivatives, 5α- androstan-3α, 7β-diol (3α-diol) and tetrahydroprogesterone. On the basis of these observations, the possibility that secretory products of normal and tumoral brain cells might be able to influence steroid metabolism occurring in the two glial cell lines previously mentioned as well as in fetal rat neurons and in neonatal rat type 1 astrocytes has been considered. To this purpose, cultures of the different cellular types have been exposed to the conditioned medium in which the other cells were grown. The results obtained indicate that: 1. Neurons are able to stimulate, in a statistically significant fashion, the formation of dihydrotestosterone (DHT), 3α-diol, and tetrahydraprogesterone (THP) in C6 cells. 2. Type 1 astrocytes, on the contrary, are unable to modify steroid metabolism in C6 cells. 3. C6 cell product(s) decrease(s) the formation of DHP in type 1 astrocytes, without modifying that of DHT. 4. C6 cells do not influence the metabolism of testosterone (T) and progesterone (P) in neurons. In conclusion, the present observations show that the conditioned medium of normal neurons is able to increase the metabolism of testosterone and progesterone occurring in a tumoral glial cell line, and that the conditioned media of the two tumoral cell lines analyzed are able to decrease the conversion of P into DHP occurring in normal type 1 astrocytes. The surprising result that these conditioned media do not alter the formation of DHT is discussed. Work is presently in progress to identify the principle(s) responsible respectively for the activating and inhibiting actions here described.",
keywords = "Astrocytes, Human astrocytoma, Neurons, Progesterone, Rat glioma, Testosterone",
author = "Melcangi, {Roberto Cosimo} and Ilaria Cavarretta and Valerio Magnaghi and Marinella Ballabio and Luciano Martini and Marcella Motta",
year = "1998",
doi = "10.1385/ENDO:8:1:65",
language = "English",
volume = "8",
pages = "65--71",
journal = "Endocrine",
issn = "1355-008X",
publisher = "Humana Press Inc.",
number = "1",

}

TY - JOUR

T1 - Crosstalk between normal and tumoral brain cells

T2 - Effect on sex steroid metabolism

AU - Melcangi, Roberto Cosimo

AU - Cavarretta, Ilaria

AU - Magnaghi, Valerio

AU - Ballabio, Marinella

AU - Martini, Luciano

AU - Motta, Marcella

PY - 1998

Y1 - 1998

N2 - The present article shows for the first time that two cell lines derived respectively from a rat glioma (C6 cell line) and from a human astrocytoma (1321N1 cell line) are able to convert testosterone and progesterone into their corresponding 5α-reduced metabolites dihydrotestosterone and dihydroprogesterone. Moreover, both cell lines are also able to convert these metabolites further into their corresponding 3α-OH derivatives, 5α- androstan-3α, 7β-diol (3α-diol) and tetrahydroprogesterone. On the basis of these observations, the possibility that secretory products of normal and tumoral brain cells might be able to influence steroid metabolism occurring in the two glial cell lines previously mentioned as well as in fetal rat neurons and in neonatal rat type 1 astrocytes has been considered. To this purpose, cultures of the different cellular types have been exposed to the conditioned medium in which the other cells were grown. The results obtained indicate that: 1. Neurons are able to stimulate, in a statistically significant fashion, the formation of dihydrotestosterone (DHT), 3α-diol, and tetrahydraprogesterone (THP) in C6 cells. 2. Type 1 astrocytes, on the contrary, are unable to modify steroid metabolism in C6 cells. 3. C6 cell product(s) decrease(s) the formation of DHP in type 1 astrocytes, without modifying that of DHT. 4. C6 cells do not influence the metabolism of testosterone (T) and progesterone (P) in neurons. In conclusion, the present observations show that the conditioned medium of normal neurons is able to increase the metabolism of testosterone and progesterone occurring in a tumoral glial cell line, and that the conditioned media of the two tumoral cell lines analyzed are able to decrease the conversion of P into DHP occurring in normal type 1 astrocytes. The surprising result that these conditioned media do not alter the formation of DHT is discussed. Work is presently in progress to identify the principle(s) responsible respectively for the activating and inhibiting actions here described.

AB - The present article shows for the first time that two cell lines derived respectively from a rat glioma (C6 cell line) and from a human astrocytoma (1321N1 cell line) are able to convert testosterone and progesterone into their corresponding 5α-reduced metabolites dihydrotestosterone and dihydroprogesterone. Moreover, both cell lines are also able to convert these metabolites further into their corresponding 3α-OH derivatives, 5α- androstan-3α, 7β-diol (3α-diol) and tetrahydroprogesterone. On the basis of these observations, the possibility that secretory products of normal and tumoral brain cells might be able to influence steroid metabolism occurring in the two glial cell lines previously mentioned as well as in fetal rat neurons and in neonatal rat type 1 astrocytes has been considered. To this purpose, cultures of the different cellular types have been exposed to the conditioned medium in which the other cells were grown. The results obtained indicate that: 1. Neurons are able to stimulate, in a statistically significant fashion, the formation of dihydrotestosterone (DHT), 3α-diol, and tetrahydraprogesterone (THP) in C6 cells. 2. Type 1 astrocytes, on the contrary, are unable to modify steroid metabolism in C6 cells. 3. C6 cell product(s) decrease(s) the formation of DHP in type 1 astrocytes, without modifying that of DHT. 4. C6 cells do not influence the metabolism of testosterone (T) and progesterone (P) in neurons. In conclusion, the present observations show that the conditioned medium of normal neurons is able to increase the metabolism of testosterone and progesterone occurring in a tumoral glial cell line, and that the conditioned media of the two tumoral cell lines analyzed are able to decrease the conversion of P into DHP occurring in normal type 1 astrocytes. The surprising result that these conditioned media do not alter the formation of DHT is discussed. Work is presently in progress to identify the principle(s) responsible respectively for the activating and inhibiting actions here described.

KW - Astrocytes

KW - Human astrocytoma

KW - Neurons

KW - Progesterone

KW - Rat glioma

KW - Testosterone

UR - http://www.scopus.com/inward/record.url?scp=0031840278&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031840278&partnerID=8YFLogxK

U2 - 10.1385/ENDO:8:1:65

DO - 10.1385/ENDO:8:1:65

M3 - Article

C2 - 9666347

AN - SCOPUS:0031840278

VL - 8

SP - 65

EP - 71

JO - Endocrine

JF - Endocrine

SN - 1355-008X

IS - 1

ER -