Crv4, a mouse model for human ataxia associated with kyphoscoliosis caused by an mRNA splicing mutation of the metabotropic glutamate receptor 1 (Grm1)

Valerio Conti, Asadollah Aghaie, Michele Cilli, Natalia Martin, Gianluca Caridi, Luca Musante, Giovanni Candiano, Maura Castagna, Alfonso Fairen, Roberto Ravazzolo, Jean Louis Guenet, Aldamaria Puliti

Research output: Contribution to journalArticle

Abstract

We describe a novel spontaneous autosomal recessive mutation, cervelet-4 (crv4), which arose in a BALB/c strain. Mice homozygous for the mutation exhibit principally a reduced body size, a congenital neurological phenotype characterized by ataxic gait and intention tremor, with no gross anomalies observed in brain or cerebellum, and skeletal anomalies. Using linkage analysis, we mapped the crv4 locus to the proximal region of chromosome 10, at the location of the Grm1 gene. Genetic complementation crosses between crv4 and Grm1 KO mice confirmed that crv4 is a new allele of Grm1. Molecular analysis of the Grm1 gene in mutant mice revealed the insertion of a 190-bp LTR fragment in intron 4. Our results also indicated that the presence of the LTR fragment caused the disruption of the Grm1 normal splicing process and complete absence of the wild-type protein. crv4 is an interesting model to extend the study of Grm1 function and the pathological effects of Grm1 deficiency in vivo.

Original languageEnglish
Pages (from-to)593-600
Number of pages8
JournalInternational Journal of Molecular Medicine
Volume18
Issue number4
Publication statusPublished - Oct 2006

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Keywords

  • Additional exon
  • Ataxia
  • Grm1
  • Kyphoscoliosis
  • Retrotransposon insertion
  • Splicing error

ASJC Scopus subject areas

  • Genetics

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