Cryo-EM structure of cardiac amyloid fibrils from an immunoglobulin light chain AL amyloidosis patient

Paolo Swuec, Francesca Lavatelli, Masayoshi Tasaki, Cristina Paissoni, Paola Rognoni, Martina Maritan, Francesca Brambilla, Paolo Milani, Pierluigi Mauri, Carlo Camilloni, Giovanni Palladini, Giampaolo Merlini, Stefano Ricagno, Martino Bolognesi

Research output: Contribution to journalArticle

Abstract

Systemic light chain amyloidosis (AL) is a life-threatening disease caused by aggregation and deposition of monoclonal immunoglobulin light chains (LC) in target organs. Severity of heart involvement is the most important factor determining prognosis. Here, we report the 4.0 Å resolution cryo-electron microscopy map and molecular model of amyloid fibrils extracted from the heart of an AL amyloidosis patient with severe amyloid cardiomyopathy. The helical fibrils are composed of a single protofilament, showing typical 4.9 Å stacking and cross-β architecture. Two distinct polypeptide stretches (total of 77 residues) from the LC variable domain (V l ) fit the fibril density. Despite V l high sequence variability, residues stabilizing the fibril core are conserved through different cardiotoxic V l , highlighting structural motifs that may be common to misfolding-prone LCs. Our data shed light on the architecture of LC amyloids, correlate amino acid sequences with fibril assembly, providing the grounds for development of innovative medicines.

Original languageEnglish
Article number1269
JournalNature Communications
Volume10
Issue number1
DOIs
Publication statusPublished - Dec 1 2019

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Immunoglobulin Light Chains
Amyloidosis
Amyloid
Light
sheds
prognosis
polypeptides
medicine
organs
Cryoelectron Microscopy
amino acids
electron microscopy
Molecular Models
assembly
Cardiomyopathies
Electron microscopy
Medicine
Amino Acid Sequence
Agglomeration
Amino Acids

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Cryo-EM structure of cardiac amyloid fibrils from an immunoglobulin light chain AL amyloidosis patient. / Swuec, Paolo; Lavatelli, Francesca; Tasaki, Masayoshi; Paissoni, Cristina; Rognoni, Paola; Maritan, Martina; Brambilla, Francesca; Milani, Paolo; Mauri, Pierluigi; Camilloni, Carlo; Palladini, Giovanni; Merlini, Giampaolo; Ricagno, Stefano; Bolognesi, Martino.

In: Nature Communications, Vol. 10, No. 1, 1269, 01.12.2019.

Research output: Contribution to journalArticle

Swuec, Paolo ; Lavatelli, Francesca ; Tasaki, Masayoshi ; Paissoni, Cristina ; Rognoni, Paola ; Maritan, Martina ; Brambilla, Francesca ; Milani, Paolo ; Mauri, Pierluigi ; Camilloni, Carlo ; Palladini, Giovanni ; Merlini, Giampaolo ; Ricagno, Stefano ; Bolognesi, Martino. / Cryo-EM structure of cardiac amyloid fibrils from an immunoglobulin light chain AL amyloidosis patient. In: Nature Communications. 2019 ; Vol. 10, No. 1.
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