Abstract
Frozen section examination (FSE) reshaped surgical pathology and is characterized by a high accuracy. Nonetheless pathologists can experience artefacts that can compromise or defer the diagnosis. We compared a commercial system, composed by a cryoembedder and a processor/stainer, to our FSE protocol. Feasibility of diagnosis as well as overall architecture, cytology, and staining, were scored under the following conditions: Traditional (liquid nitrogen freezing and manual staining), Only-Presto (liquid nitrogen freezing and commercial processor/stainer), Only-PrestoCHILL (cryoembedder and manual staining), and PrestoSystem (cryoembedder and processor/stainer). Scores were compared across the different experimental conditions. PrestoSystem had significantly higher scores than Traditional, Only-Presto or Only-PrestoCHILL in all categories (Wilcoxon test; all P-value <.001); similarly, also Only-Presto and Only-PrestoChill had significantly higher scores than Traditional in all categories. Only-PrestoCHILL had significantly higher scores than Only-Presto in Cytology and Architecture. In conclusion the control of pre-analytical variables provided reproducible results, of a higher quality.
| Original language | English |
|---|---|
| Pages (from-to) | 761-764 |
| Number of pages | 4 |
| Journal | Acta Histochemica |
| Volume | 121 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Aug 2019 |
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Keywords
- Automation
- Clinical decision-making
- Diagnostic techniques
- Frozen sections
- Surgical
ASJC Scopus subject areas
- Histology
- Cell Biology
Cite this
Cryoembedder, automatic processor/stainer, liquid nitrogen freezing, and manual staining for frozen section examination : A comparative study. / Renne, Salvatore Lorenzo; Redaelli, Silvia; Paolini, Biagio.
In: Acta Histochemica, Vol. 121, No. 6, 08.2019, p. 761-764.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cryoembedder, automatic processor/stainer, liquid nitrogen freezing, and manual staining for frozen section examination
T2 - A comparative study
AU - Renne, Salvatore Lorenzo
AU - Redaelli, Silvia
AU - Paolini, Biagio
PY - 2019/8
Y1 - 2019/8
N2 - Frozen section examination (FSE) reshaped surgical pathology and is characterized by a high accuracy. Nonetheless pathologists can experience artefacts that can compromise or defer the diagnosis. We compared a commercial system, composed by a cryoembedder and a processor/stainer, to our FSE protocol. Feasibility of diagnosis as well as overall architecture, cytology, and staining, were scored under the following conditions: Traditional (liquid nitrogen freezing and manual staining), Only-Presto (liquid nitrogen freezing and commercial processor/stainer), Only-PrestoCHILL (cryoembedder and manual staining), and PrestoSystem (cryoembedder and processor/stainer). Scores were compared across the different experimental conditions. PrestoSystem had significantly higher scores than Traditional, Only-Presto or Only-PrestoCHILL in all categories (Wilcoxon test; all P-value <.001); similarly, also Only-Presto and Only-PrestoChill had significantly higher scores than Traditional in all categories. Only-PrestoCHILL had significantly higher scores than Only-Presto in Cytology and Architecture. In conclusion the control of pre-analytical variables provided reproducible results, of a higher quality.
AB - Frozen section examination (FSE) reshaped surgical pathology and is characterized by a high accuracy. Nonetheless pathologists can experience artefacts that can compromise or defer the diagnosis. We compared a commercial system, composed by a cryoembedder and a processor/stainer, to our FSE protocol. Feasibility of diagnosis as well as overall architecture, cytology, and staining, were scored under the following conditions: Traditional (liquid nitrogen freezing and manual staining), Only-Presto (liquid nitrogen freezing and commercial processor/stainer), Only-PrestoCHILL (cryoembedder and manual staining), and PrestoSystem (cryoembedder and processor/stainer). Scores were compared across the different experimental conditions. PrestoSystem had significantly higher scores than Traditional, Only-Presto or Only-PrestoCHILL in all categories (Wilcoxon test; all P-value <.001); similarly, also Only-Presto and Only-PrestoChill had significantly higher scores than Traditional in all categories. Only-PrestoCHILL had significantly higher scores than Only-Presto in Cytology and Architecture. In conclusion the control of pre-analytical variables provided reproducible results, of a higher quality.
KW - Automation
KW - Clinical decision-making
KW - Diagnostic techniques
KW - Frozen sections
KW - Surgical
UR - http://www.scopus.com/inward/record.url?scp=85065127179&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85065127179&partnerID=8YFLogxK
U2 - 10.1016/j.acthis.2019.05.002
DO - 10.1016/j.acthis.2019.05.002
M3 - Article
C2 - 31078257
AN - SCOPUS:85065127179
VL - 121
SP - 761
EP - 764
JO - Acta Histochemica
JF - Acta Histochemica
SN - 0065-1281
IS - 6
ER -