Cryoembedder, automatic processor/stainer, liquid nitrogen freezing, and manual staining for frozen section examination: A comparative study

Salvatore Lorenzo Renne, Silvia Redaelli, Biagio Paolini

Research output: Contribution to journalArticle

Abstract

Frozen section examination (FSE) reshaped surgical pathology and is characterized by a high accuracy. Nonetheless pathologists can experience artefacts that can compromise or defer the diagnosis. We compared a commercial system, composed by a cryoembedder and a processor/stainer, to our FSE protocol. Feasibility of diagnosis as well as overall architecture, cytology, and staining, were scored under the following conditions: Traditional (liquid nitrogen freezing and manual staining), Only-Presto (liquid nitrogen freezing and commercial processor/stainer), Only-PrestoCHILL (cryoembedder and manual staining), and PrestoSystem (cryoembedder and processor/stainer). Scores were compared across the different experimental conditions. PrestoSystem had significantly higher scores than Traditional, Only-Presto or Only-PrestoCHILL in all categories (Wilcoxon test; all P-value <.001); similarly, also Only-Presto and Only-PrestoChill had significantly higher scores than Traditional in all categories. Only-PrestoCHILL had significantly higher scores than Only-Presto in Cytology and Architecture. In conclusion the control of pre-analytical variables provided reproducible results, of a higher quality.

Original languageEnglish
Pages (from-to)761-764
Number of pages4
JournalActa Histochemica
Volume121
Issue number6
DOIs
Publication statusPublished - Aug 2019

Fingerprint

Frozen Sections
Freezing
Nitrogen
Staining and Labeling
Cell Biology
Surgical Pathology
Artifacts

Keywords

  • Automation
  • Clinical decision-making
  • Diagnostic techniques
  • Frozen sections
  • Surgical

ASJC Scopus subject areas

  • Histology
  • Cell Biology

Cite this

Cryoembedder, automatic processor/stainer, liquid nitrogen freezing, and manual staining for frozen section examination : A comparative study. / Renne, Salvatore Lorenzo; Redaelli, Silvia; Paolini, Biagio.

In: Acta Histochemica, Vol. 121, No. 6, 08.2019, p. 761-764.

Research output: Contribution to journalArticle

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