Crystal structure of mouse CD1d bound to the self ligand phosphatidylcholine: A molecular basis for NKT cell activation

Barbara Giabbai, Stèphane Sidobre, M. D Max Crispin, Yovan Sanchez-Ruìz, Angela Bachi, Mitchell Kronenberg, Ian A. Wilson, Massimo Degano

Research output: Contribution to journalArticlepeer-review

Abstract

NKT cells are immunoregulatory lymphocytes whose activation is triggered by the recognition of lipid Ags in the context of the CD1d molecules by the TCR. In this study we present the crystal structure to 2.8 Å of mouse CD1d bound to phosphatidylcholine. The interactions between the ligand acyl chains and the CD1d molecule define the structural and chemical requirements for the binding of lipid Ags to CD1d. The orientation of the polar headgroup toward the C terminus of the α1 helix provides a rationale for the structural basis for the observed Vα chain bias in invariant NKT cells. The contribution of the ligand to the protein surface suggests a likely mode of recognition of lipid Ags by the NKT cell TCR.

Original languageEnglish
Pages (from-to)977-984
Number of pages8
JournalJournal of Immunology
Volume175
Issue number2
Publication statusPublished - Jul 15 2005

ASJC Scopus subject areas

  • Immunology

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