Crystal structures of human Ero1α reveal the mechanisms of regulated and targeted oxidation of PDI

Kenji Inaba, Shoji Masui, Hiroka Iida, Stefano Vavassori, Roberto Sitia, Mamoru Suzuki

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

In the endoplasmic reticulum (ER) of eukaryotic cells, Ero1 flavoenzymes promote oxidative protein folding through protein disulphide isomerase (PDI), generating reactive oxygen species (hydrogen peroxide) as byproducts. Therefore, Ero1 activity must be strictly regulated to avoid futile oxidation cycles in the ER. Although regulatory mechanisms restraining Ero1α activity ensure that not all PDIs are oxidized, its specificity towards PDI could allow other resident oxidoreductases to remain reduced and competent to carry out isomerization and reduction of protein substrates. In this study, crystal structures of human Ero1α were solved in its hyperactive and inactive forms. Our findings reveal that human Ero1α modulates its oxidative activity by properly positioning regulatory cysteines within an intrinsically flexible loop, and by fine-tuning the electron shuttle ability of the loop through disulphide rearrangements. Specific PDI targeting is guaranteed by electrostatic and hydrophobic interactions of Ero1α with the PDI b′-domain through its substrate-binding pocket. These results reveal the molecular basis of the regulation and specificity of protein disulphide formation in human cells.

Original languageEnglish
Pages (from-to)3330-3343
Number of pages14
JournalEMBO Journal
Volume29
Issue number19
DOIs
Publication statusPublished - Oct 6 2010

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Protein Disulfide-Isomerases
Crystal structure
Oxidation
Endoplasmic Reticulum
Disulfides
Substrate Cycling
Protein folding
Protein Folding
Eukaryotic Cells
Substrates
Isomerization
Static Electricity
Hydrophobic and Hydrophilic Interactions
Hydrogen Peroxide
Cysteine
Byproducts
Electrostatics
Reactive Oxygen Species
Oxidoreductases
Proteins

Keywords

  • disulphide bond
  • ER quality control
  • human Ero1a
  • redox homeostasis
  • X-ray crystal structure analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)
  • Medicine(all)

Cite this

Crystal structures of human Ero1α reveal the mechanisms of regulated and targeted oxidation of PDI. / Inaba, Kenji; Masui, Shoji; Iida, Hiroka; Vavassori, Stefano; Sitia, Roberto; Suzuki, Mamoru.

In: EMBO Journal, Vol. 29, No. 19, 06.10.2010, p. 3330-3343.

Research output: Contribution to journalArticle

Inaba, Kenji ; Masui, Shoji ; Iida, Hiroka ; Vavassori, Stefano ; Sitia, Roberto ; Suzuki, Mamoru. / Crystal structures of human Ero1α reveal the mechanisms of regulated and targeted oxidation of PDI. In: EMBO Journal. 2010 ; Vol. 29, No. 19. pp. 3330-3343.
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